Investigation of new treatment strategy for uterine carcinosarcoma by using cancer tissue originated spheroid

• Project/Area Number: 25462591
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Osaka University
• Principal Investigator: FUJITA MASAMI 大阪大学, 医学(系)研究科(研究院), 招へい准教授 (60303963)
• Co-Investigator(Kenkyū-buntansha): Yoshino Kiyoshi 大阪大学, 大学院医学系研究科, 准教授 (90362730) ; Ueda Yutaka 大阪大学, 大学院医学系研究科, 助教 (10346215) ; Kobayashi Eiji 大阪大学, 大学院医学系研究科, 助教 (50614773) ; Kimura Toshihiro 大阪大学, 大学院医学系研究科, 招へい教員 (90584524)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 婦人科腫瘍 / 子宮体部癌内腫 / 子宮体部癌肉腫 / CTOS

Outline of Final Research Achievements

Advanced uterine carcinosarcoma is difficult to cure and the standard treatment is not determined. We searched if we can establish primary culture of uterine carcinosarcoma by cancer tissue-originated spheroid (CTOS) method and if we can use drug screening kit for uterine carcinosarcoma by using CTOS method. One of the results is that we established primary culture of uterine carcinosarcoma by CTOS method. And the other result is that we found we can screen effective drugs for uterine carcinosarcoma by CTOS method and drug screening kit. We could not proceed to animal model. However we showed personalized medicine is also feasible by CTOS methods.

Research Products

• [Journal Article] Plasma nlembrane proteonlics identifies bone marrow stromal antigen 2 as a potential therapeutic target in endometrial cancer (2013)
  • Author(s): Yokoyama T, Enomoto T, Serada S, Morimoto A, Matsuzaki S, Ueda Y, Yoshino K, Fujita M, Kyo S, Iwahori K, Fujimoto M, Kimura T, Naka T
  • Journal Title: Int J Cancer Volume: 132(2) Issue: 2 Pages: 472-84
  • DOI: 10.1002/ijc.27679
  • Peer Reviewed
• [Presentation] Annexin A4-conferred platinum resistance is mediated by the copper transporter ATP7A (2013)
  • Author(s): Matsuzaki, S. Morimoto, A. Serada, S. Yokoyama, T. Kimura, T. Kobayashi, E. Ueda, Y. Yoshino, K. Fujita, M. Enomoto, T. Naka, T. Kimura, T.
  • Organizer: The 104th Annual Meeting American Association for Cancer Research
  • Place of Presentation: Washington DC, U.S.A.
• [Presentation] Annexin A4の発現は銅のトランスポーターでありプラチナの排出に関与すると知られるATP7Aを介して，耐性獲得に関与する (2013)
  • Author(s): 松崎慎哉 森本晶子 横山拓平 小林栄仁 木村敏啓 上田豊 吉野潔 藤田征巳 榎本隆之 木村正 仲哲治
  • Organizer: 第65回日本産科婦人科学会学術講演会
  • Place of Presentation: 札幌
• [Presentation] プラチナ耐性に関与するAnnexin A4の機能領域解析 (2013)
  • Author(s): 森本晶子 榎本隆之 松崎慎哉 横山拓平 小林栄仁 木村敏啓 上田豊 吉野潔 藤田征巳 仲哲治 木村正
  • Organizer: 第65回日本産科婦人科学会学術講演会
  • Place of Presentation: 札幌
• [Presentation] Annexin A4の機能領域解析を用いた癌細胞プラチナ耐性機構の解明 (2013)
  • Author(s): 森本晶子 榎本隆之 世良田聡 木村敏啓 上田豊 藤田征巳 仲哲治 木村正
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜