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Novel oligomannose liposome-DNA complex DNA vaccination efficiently evokes anti-HPV E6 and E7 CTL responses.

Research Project

Project/Area Number 25462599
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionSapporo Medical University

Principal Investigator

Saito Tsuyoshi  札幌医科大学, 医学部, 教授 (90145566)

Co-Investigator(Kenkyū-buntansha) Iwasaki Masahiro  札幌医科大学, 医学部, 准教授 (20516504)
Tanaka Ryoichi  札幌医科大学, 医学部, 講師 (60448602)
Sato Noriyuki  札幌医科大学, その他, 名誉教授 (50158937)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords免疫療法 / 子宮頸癌 / HPV / ワクチン / ペプチドワクチン / CTL
Outline of Final Research Achievements

The aim of this study was to establish an efficient HPV type 16-targeting cancer immunotherapy. HPV16-derived viral proteins E6 and E7 are expressed in cancerous cells through the progression of the disease and have a role in carcinogenesis but are not expressed in normal cells. In this study, we generated a novel HPV 16 E6 and E7 gene plasmid containing oligomannose liposomes (OML-HPV). We compared the cytotoxic T lymphocyte (CTL) induction efficiency of OML-HPV and that of standard liposome-HPV16 E6 and E7 DNA complex. HPV16 E6-specific CTLs could be generated from HPV 16-positive cervical carcinoma patient's peripheral blood mononuclear cells by stimulating OML-HPV, but could not by stimulating standard liposome-HPV 16 E6, E7 DNA complex. These results indicate that OML-HPV is a more effective approach than DNA vaccination using standard liposomes, and that a novel HLA-A24-restricted peptide, E6 66-74, might be a suitable target of cervical cancer immunotherapy.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (13 results)

All 2016 2015 2013

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 5 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results)

  • [Journal Article] Brother of the regulator of the imprinted site (BORIS) variant subfamily 6 is involved in cervical cancer stemness and can be a target of immunotherapy.2016

    • Author(s)
      Asano T, Hirohashi Y, Torigoe T, Mariya T, Horibe R, Kuroda T, Tabuchi Y, Saijo H, Yasuda K, Mizuuchi M, Takahashi A, Asanuma H, Hasegawa T, Saito T, Sato N.
    • Journal Title

      Oncotarget

      Volume: 7 Issue: 10 Pages: 11223-11237

    • DOI

      10.18632/oncotarget.7165

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Analysis of the expression and localization of tight junction transmembrane proteins, claudin-1, -4, -7, occludin and JAM-A, in human cervical adenocarcinoma.2016

    • Author(s)
      Saito T
    • Journal Title

      Histol Histopathol.

      Volume: Epub ahead of print Pages: 1-34

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Obstetrical prognosis of patients with cervical intraepithelial neoplasia (CIN) after "coin-shaped" conization.2016

    • Author(s)
      Saito T
    • Journal Title

      Arch Gynecol Obstet.

      Volume: 293 Issue: 3 Pages: 651-657

    • DOI

      10.1007/s00404-015-3860-5

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] BAG3 increases the invasiveness of uterine corpus carcinoma cells by suppressing miR-29b and enhancing MMP2 expression.2015

    • Author(s)
      Saito T
    • Journal Title

      Oncol Rep

      Volume: 33 Pages: 2613-21

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] HLA class I as a predictor of clinical prognosis and CTL infiltration as a predictor of chemosensitivity in ovarian cancer2015

    • Author(s)
      Saito T Sato N
    • Journal Title

      Oncoimmunology

      Volume: 23

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Excessive androgen exposure in female-to-male transsexual persons of reproductive age induces hyperplasia of the ovarian cortex and stroma but not polycystic ovary morphology.2013

    • Author(s)
      Saito T
    • Journal Title

      Hum Reprod

      Volume: 28 Issue: 2 Pages: 453-61

    • DOI

      10.1093/humrep/des385

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Assisted reproductive technique increases the risk of placental polyp.2013

    • Author(s)
      Baba T, Endo T, Ikeda K, Shimizu A, Morishita M, Kuno Y, Honnma H, Kiya T, Ishioka S, Saito T.
    • Journal Title

      Gynecol Endocrinol

      Volume: 29 Issue: 6 Pages: 611-614

    • DOI

      10.3109/09513590.2013.788636

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Importance of uterine cervical cerclage to maintain a successful pregnancy for patients who undergo vaginal radical trachelectomy.2013

    • Author(s)
      Saito T
    • Journal Title

      Int J Clin Oncol.

      Volume: [Epub ahead of print]

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Identification of Cancer stem-like cells from epithelial ovarian cancer and investigation of Matrix Metalloproteinase-10 gene as a regulatory part in ovarian cancer stem-like cells2015

    • Author(s)
      Saito T Sato N
    • Organizer
      XXI FIGO World Congress of Gynecology and Obstetrics
    • Place of Presentation
      Vancuver Canada
    • Year and Date
      2015-11-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Brother of the regulator of the imprinted site (BORIS) variant subfamily 6 is involved in cervical cancer stemness and can be a target of immunotherapy.2015

    • Author(s)
      Saito T
    • Organizer
      International Meeting of The European Society of Gynaecological Oncology
    • Place of Presentation
      Nice France
    • Year and Date
      2015-10-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 〈ワークショップ〉・ 正常ヒト子宮内膜上皮および子宮内膜癌細胞株Sawanoにおける性ホルモンによるタイト結合の調節機構2013

    • Author(s)
      齋藤 豪
    • Organizer
      第45回日本臨床分子形態学会総会・学術集会
    • Place of Presentation
      福岡市
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Isolation and analysis of cancer stem-like cells from primary epithelial ovarian cancer2013

    • Author(s)
      齋藤 豪
    • Organizer
      公益社団法人日本産科婦人科学会第65回学術講演会
    • Place of Presentation
      札幌市
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] BAG3遺伝子が卵巣癌の治療効果に及ぼす影響~microRNAによる調節を検討する~2013

    • Author(s)
      齋藤 豪、岩崎雅宏
    • Organizer
      公益社団法人日本産科婦人科学会第65回学術講演会
    • Place of Presentation
      札幌市
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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