Investigation of the role of glycolipids in anticancer drug resistance of ovarian carcinoma derived cells

• Project/Area Number: 25462610
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: tanaka kyoko 慶應義塾大学, 医学部, 講師 (10286536)
• Co-Investigator(Kenkyū-buntansha): IWAMORI Masao 近畿大学, 理工学部, 講師 (90110022)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 硫酸化糖脂質 / 子宮内膜癌 / 高分化・低分化型 / 粘液性卵巣癌 / 抗がん剤感受性 / 抗がん剤耐性 / 遺伝子導入 / 糖脂質代謝経路 / 細胞膜 / 糖脂質 / 糖鎖特異的抗体 / 免疫検出 / ガングリオシド / ルイス抗原 / 抗がん剤 / ABC-トランスポーター

Outline of Final Research Achievements

We determine the glycolipid　expressions in endometrial carcinoma and ovarian carcinoma　tissues. Sulfatide did not expressed in endometrial carcinoma in poorly differentiated type, but it expressed in well-differentiated type. Ovarian mucinous adenocarcinoma tissues preferentially contained　sulfatide which was more than 5 times of high concentration of well-differentiated endometrial carcinoma. Sulfotransferase gene was transfected in SNG-II cells, which did not contain sulfated glycolipid. The transfected cells revealed more resistant properties toward anticancer drugs than SNG-II cells. It indicated that Sulfatide is concerned with sensitivity or resistance in an anticancer drugs.

Research Products

• [Journal Article] Pregnancy Outcomes After Abdominal Radical Trachelectomy for Early-Stage Cervical Cancer: A 13-Year Experience in a Single Tertiary-Care Center (2016)
  • Author(s): Kasuga Y, Nishio H, Miyakoshi K, Sato S, Sugiyama J, Matsumoto T, Tanaka K, Ochiai D, Minegishi K, Hamatani T, Iwata T, Morisada T, Nakamura M, Fujii T, Kuji N, Aoki D, Tanaka M
  • Journal Title: Int J Gynecol Cancer Volume: 26 Issue: 1 Pages: 163-168
  • DOI: 10.1097/igc.0000000000000571
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Absence of lactobacilli containing glycolipids with the α-galactose epitope and the enhanced fucosylation of a receptor glycolipid GA1 in the digestive tracts of immune-deficient scid mice. (2015)
  • Author(s): Iwamori M, Tanaka K, Adachi S, Aoki D, Nomura T.
  • Journal Title: J Biochem Volume: pii Issue: 1 Pages: 73-82
  • DOI: 10.1093/jb/mvv021
  • Peer Reviewed
• [Journal Article] Expression of sulfatide and sulfated lactosylceramide among histological types of human ovarian carcinomas. (2015)
  • Author(s): Tanaka K, Mikami M, Aoki D, Kiguchi K, Ishiwata I, Iwamori M.
  • Journal Title: Hum Cell Volume: 28 Issue: 1 Pages: 37-43
  • DOI: 10.1007/s13577-014-0100-4
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] ヒト子宮頸癌由来細胞のGM2糖鎖を持つガングリオシドの合成酵素遺伝子 (2015)
  • Author(s): 田中京子、宮澤昌樹、三上幹男、青木大輔、木口一成、岩森正男
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] 糖脂質硫酸基転移酵素遺伝子の導入によるヒト子宮内膜癌細胞の表現形質の変化 (2015)
  • Author(s): 本田葵、田中京子、青木大輔、岩森正男
  • Organizer: 第62回日本生化学会近畿支部例会
  • Place of Presentation: 立命館大学（滋賀県草津市）
  • Year and Date: 2015-05-16