Analysis of molecular mechanism of lymphatic metastasis of gynecologic malignant tumor
| Project/Area Number |
25462613
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUJINO Akihiro 慶應義塾大学, 医学部, 講師 (50306726)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | リンパ管内皮細胞 / 婦人科悪性腫瘍 / リンパ管腫 |
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| Outline of Final Research Achievements |
We have been analyzing molecular interaction between tumor cells and lymphatic endothelium using lymphatic endothelial cell (LEC) line and gynecologic cancer cell line. After establishment of immortalization and cultivation of LEC, we observed elevated expression of cell adhesion molecules on LEC by adding inflammatory cytokine, TNFα. The lymphatic vessels are supposed to have a certain role in lymphatic metastasis by promoting cell adhesion. It was confirmed that a uterine cervical cancer cell, SKG-2 formed cell spheroid in a three-dimensional culture dish. It is suggested that this cell spheroid possesses the biological character mimicking the gynecologic tumor in cancer microenvironment.
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Report
(3 results)
Research Products
(1 results)
