Molecuar mechanisms and new therapeutic strategies for tinnitus

• Project/Area Number: 25462639
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Nara Medical University (2015); Osaka University (2013)
• Principal Investigator: Kitahara Tadashi 奈良県立医科大学, 医学部, 教授 (30343255)
• Co-Investigator(Kenkyū-buntansha): IMAI TAKAO 大阪大学, 医学(系)研究科(研究院), 講師 (80570663) ; OTA YUMI 大阪大学, 医学(系)研究科(研究院), 助教 (00598401) ; MORIHANA TETSUO 大阪大学, 医学(系)研究科(研究院), 助教 (80634170)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 耳鳴 / 逃避行動 / TRPファミリー / 侵害受容体 / サリチル酸 / 動物モデル / 神経栄養因子 / 内耳障害

Outline of Final Research Achievements

Due to moving from Osaka University to Nara Medical University, we established the animal behavioral model for tinnitus again. Salicylate-induced tinnitus in rats could be visualized by behavioral and molecular experiments. Behavioral experiments were performed using an active avoidance task and molecular experiments were performed using a nociceptive receptor, transient receptor potential cation channel superfamily V (TRPV) in the rat auditory pathway. By means of these tinnitus visualization experiments, capsazepine, a specific antagonist of TRPV1, could be one of candidates to block tinnitus pathway and cure tinnitus. Further additional experiments are required.

Research Products

• [Journal Article] Negative prognostic factors for psychological condition in patients with audio-vestibular diseases (2016)
  • Author(s): Sakagami-M, Kitahara-T, Okayasu-T, Yamashita-A, Hasukawa-A, Ota-I, Yamanaka-T
  • Journal Title: Auris Nasus Larynx Volume: in press
  • NAID: 130005773904
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Surgical results and psychological status in patients with intractable Meniere’s disease (2016)
  • Author(s): Yokota-Y, Kitahara-T, Sakagami-M, Ito-T, Kimura-T, Okayasu-T, Yamashita-A, Yamanaka-T
  • Journal Title: Auris Nasus Larynx Volume: in press
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Endolymphatic space size in patients with Meniere’s disease and healthy controls (2016)
  • Author(s): Ito-T, Kitahara-T, Inui-H, Miyasaka, Kichikawa, Ota-I, Nario-K, Sawai-Matsumura, Yamanaka-T
  • Journal Title: Acta Otolaryngologica Volume: in press
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cartilage conduction is characterized by vibrations of the cartilaginous portion of the ear canal (2015)
  • Author(s): Nishimura-T, Hosoi-H, Saito-O, Miyamae-R, Shimokura-R, Yamanaka-T, Kitahara-T, Levitt-H
  • Journal Title: PLOS ONE Volume: 10
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] NKCCs in the fibrocytes of the spiral ligament are silent on the unidirectional K+ transport that controls the electrochemical properties in the mammalian cochlea (2015)
  • Author(s): Yoshida-T, Nin-F, Ogata-G, Uetsuka-S, Kitahara-T, Inohara-H, Akazawa-K, Komune-S, Kurachi-Y, Hibino-H
  • Journal Title: Pflugers Arch Volume: 467 Pages: 1577-1589
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular architecture of the stria vascularis membrane transport system, which is essential for physiological functions of the mammalian cochlea (2015)
  • Author(s): Uetsuka-S, Ogata-G, Nagamori-S, Isozumi-N, Nin-F, Yoshida-T, Komune-S, Kitahara-T, Kikkawa-Y, Inohara-H, Kanai-Y, Hibino-H
  • Journal Title: Eur. J Neurosci Volume: 42 Pages: 1984-2002
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Residual tinnitus after the medical treatment of sudden deafness (2013)
  • Author(s): Michiba-T, Kitahara-T, Hikita-Watanabe-N, Fukushima-M, Ozono-Y, Imai-R, Inohara-H
  • Journal Title: Auris Nasus Larynx Volume: 40 Pages: 162-166
  • Peer Reviewed
• [Journal Article] Functional expression of TRPV1 and TRPA1 in rat vestibular ganglia (2013)
  • Author(s): Kamakura-T, Ishida-Y, Nakamura-Y, Yamada-T, Kitahara-T, Takimoto-Y, Horii-A, Uno-A, Imai-T, Okazaki-S, Inohara-H, Shimada-S
  • Journal Title: Neurosci Lett Volume: 552 Pages: 92-97
  • Peer Reviewed
• [Journal Article] Psychological condition in patients with intractable Meniere's disease (2013)
  • Author(s): Furukawa-M, Kitahara-T, Horii-A, Uno-A, Imai-T, Ohta-Y, Morihana-T, Inohara-H, Mishiro-Y, Sakagami-M
  • Journal Title: Acta Otolaryngologica Volume: 133 Pages: 584-589
  • Peer Reviewed
• [Presentation] 突発性難聴聴力固定後の耳鳴経過 (2015)
  • Author(s): 北原 糺、山下哲範、伊藤妙子、阪上雅治、道場隆博
  • Organizer: 第1回耳鳴・難聴研究会
  • Place of Presentation: 東京都
  • Year and Date: 2015-07-11
• [Presentation] 耳鳴を可視化する (2015)
  • Author(s): 北原 糺、山下哲範、伊藤妙子、阪上雅治、山中敏彰
  • Organizer: 第30回奈良脳神経ネットワーク研究会
  • Place of Presentation: 奈良県
  • Year and Date: 2015-07-10
• [Presentation] Functional analysis of TRPA1 and TRPV1 channels in rat vestibular ganglia (2014)
  • Author(s): Kamakura-T, Ishida-Y, Nakamura-Y, Yamada-T, Kitahara-T, Takimoto-Y, Horii-A, Uno-A, Imai-T, Okazaki-S, Inohara-H, Shimada-S
  • Organizer: 36th Annual ARO Midwinter Meeting
  • Place of Presentation: Baltimore, Maryland, USA