Protective effects of bone marrow-derived mononuclear cells in young gerbils on ischemic cochlear damage

• Project/Area Number: 25462645
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Ehime University
• Principal Investigator: Sakanaka Masahiro 愛媛大学, 医学(系)研究科(研究院), 教授 (60170601)
• Co-Investigator(Kenkyū-buntansha): Gyo Kiyofumi 愛媛大学, 大学院医学系研究科, 教授 (00108383) ; Sumiyoshi Maho 愛媛大学, 大学院医学系研究科, 助教 (60444767) ; Hata Ryuji 藤田保健衛生大学, 医学部, 教授 (90258153) ; Ju Pensyan 愛媛大学, 大学院医学系研究科, 助教 (40380216)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 内耳虚血 / 幹細胞 / 再生医療 / 虚血性難聴 / 骨髄単核球

Outline of Final Research Achievements

Circulating bone marrow-derived endothelial progenitor cells have been reported to be originated from bone marrow-derived mononuclear cells (=BMCs) and implicated in homeostasis of the microvasculature. Decreased levels of circulating endothelial progenitor cells, associated with aging correlate with poor clinical outcomes in a range of cardiovascular diseases. Herein, we transplanted BMCs from young animals (=yBMCs) or aged animals (=aBMCs) into aged gerbils. Aged gerbils transplanted with yBMCs after ischemia significantly reduced ischemic cochlear damage comparing with animals transplanted with aBMCs. Furthermore, aged gerbils transplanted with yBMCs depleted of CD34 positive cells after ischemia also reduced ischemic cochlear damage. Although further investigations must be required to elucidate the fundamental mechanism underlying senescence of the vasculature, our results shows the possibility that factors other than CD34 have protective effects on ischemic cochlear damage.

Research Products

• [Journal Article] Patients that have Undergone Hemodialysis Exhibit Lower Amyloid Deposition in the Brain: Evidence Supporting a Therapeutic Strategy for Alzheimer's Disease by Removal of Blood Amyloid. (2016)
  • Author(s): Sakai K, Senda T, Hata R, Kuroda M, Hasegawa M, Kato M, Abe M, Kawaguchi K, Nakai S, Hiki Y, Yuzawa Y, Kitaguchi N
  • Journal Title: J Alzheimers Dis. Volume: 51 Issue: 4 Pages: 997-1002
  • DOI: 10.3233/jad-151139
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Intravenous Infusion of Intravenous Infusion of Ginsenoside Rb1 Ameliorates Compressive Spinal Cord Injury through Upregulation of Bcl-xL and VEGF (2015)
  • Author(s): P Zhu, R Hata, K Nakata, F Cao, K Samukawa, H Fujita and M Sakanaka
  • Journal Title: J Neurol Neurother Volume: 2 Pages: 1-6
  • Peer Reviewed / Open Access / Acknowledgement Compliant