| Project/Area Number |
25462659
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
Saito Shinji 筑波大学, 医学医療系, 准教授 (50195989)
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| Co-Investigator(Kenkyū-buntansha) |
OHNIWA Ryosuke 筑波大学, 医学医療系, 准教授 (30447883)
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| Co-Investigator(Renkei-kenkyūsha) |
YOKOTA Shinichi 札幌医科大学, 医学部, 教授 (10325863)
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| Research Collaborator |
YANASE Haruka 筑波大学, 人間総合科学研究科・フロンティア医科学専攻, 大学院生
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 黄色ブドウ球菌 / 鼻腔上皮細胞 / アレルギー性鼻炎 / 常在細菌叢 / アレルギー応答修飾 / 気相液相界面培養 / アレルギー応答調節 |
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| Outline of Final Research Achievements |
We hypothesize that the interaction between host nasal cavity epithelium cell and the habitual Staphylococcus aureus influence the pathophysiologic condition of allergic rhinitis. To elucidate this, we tried construction of in vitro nasal epithelium model. A long-term air-liquid interface culture induces the differentiation of human nasal epithelial cell line. After long-term air-liquid interface culture but not normal liquid culture, tricellulin, one of tight junction proteins, is localized at the cell to cell junction. It is expected that a long-term air-liquid interface culture of human nasal epithelial cell line becomes a useful in vitro model of normal human nasal epithelium.
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