Analysis of abnormal micro RNA expression associated with HPV-related head and neck carcinogenesis and prognosis using microarray.

• Project/Area Number: 25462673
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Tohoku University
• Principal Investigator: Ishida Eiichi 東北大学, 医学(系)研究科(研究院), 非常勤講師 (50644811)
• Co-Investigator(Kenkyū-buntansha): Ogawa TAKENORI 東北大学, 大学病院, 講師 (50375060) ; HORII Akira 東北大学, 医学(系)研究科(研究院), 教授 (40249983) ; SAIKI Yuriko 東北大学, 医学(系)研究科(研究院), 助教 (80311223)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: HPV / ヒトパピローマウイルス / 鼻副鼻腔内反性乳頭腫 / 鼻副鼻腔癌 / 原発不明頭頸部癌 / 中咽頭癌 / マイクロＲＮＡ / ヒト乳頭腫ウイルス / マイクロRNA / 頭頸部癌

Outline of Final Research Achievements

In recent years, the prevalence of human papilloma virus (HPV)-related oropharyngeal cancer is increasing in European countries and the United States, and it is also becoming increasingly relevant in clinical practice in Japan. In response to this, we widely elucidated the association of HPV in Japanease head and neck cancer cases, such as oropharyngeal cancer and sinonasal cancer coexistent with inverted papilloma. In addition, as a result of the micro RNA microarray using extracted RNA from formalin-fixed paraffin-embedded oropharyngeal cancer specimens, we revealed micro-RNAs showing specific changes in expression in Japanese HPV-related oropharyngeal cancer cases. These could be implemented as a new prognostic or carcinogenic biomarker of HPV-related oropharyngeal cancer in the future.

Research Products

• [Journal Article] Identification of the murine H-2D(b) and human HLA-A*0201 MHC class I-restricted HPV6 E7-specific cytotoxic T lymphocyte epitopes (2016)
  • Author(s): Peng S, Mattox A, Best SR, Barbu AM, Burns JA, Akpeng B, Jeang J, Yang B, Ishida E, Hung CF, Wu TC, Pai SI
  • Journal Title: Cancer Immunology, Immunotherapy Volume: 65 Issue: 3 Pages: 261-271
  • DOI: 10.1007/s00262-016-1793-x
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Acquisition of chemoresistance to gemcitabine is induced by a loss-of-function missense mutation of DCK. (2015)
  • Author(s): Nakano T, Saiki Y, Kudo C, Hirayama A, Mizuguchi Y, Fujiwara S, Soga T, Sunamura M, Matsumura N, Motoi F, Unno M, Horii A.
  • Journal Title: Biochem Biophys Res Commun Volume: 464 Issue: 4 Pages: 1084-1089
  • DOI: 10.1016/j.bbrc.2015.07.080
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Endoscopic balloon dilatation for pharyngo-upper esophageal stricture after treatment of head and neck cancer (2015)
  • Author(s): Maejima R, Iijima K, Koike T, Ara N, Uno K, Hatta W, Ogawa T, Watanabe K, Katori Y, Shimosegawa T
  • Journal Title: Digestive Endoscopy Volume: 27 Issue: 3 Pages: 310-316
  • DOI: 10.1111/den.12345
  • Peer Reviewed
• [Journal Article] An integrated genome-wide approach to discover deregulated microRNAs in non-small cell lung cancer: Clinical significance of miR-23b-3p deregulation (2015)
  • Author(s): Begum S, Hayashi M, Ogawa T, Jabboure FJ, Brait M, Izumchenko E, Tabak S, Ahrendt SA, Westra WH, Koch W, Sidransky D, Hoque MO
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 13236-13236
  • DOI: 10.1038/srep13236
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effect of HMB/Arg/Gln on the prevention of radiation dermatitis in head and neck cancer patients treated with concurrent chemoradiotherapy. (2014)
  • Author(s): T. Imai, K. Matsuura, Y. Asada, S. Sagai, K. Katagiri, E. Ishida, D. Saito, R. Sadayasu, H. Wada and S. Saijo
  • Journal Title: Jpn J Clin Oncol Volume: 44 Issue: 5 Pages: 422-427
  • DOI: 10.1093/jjco/hyu027
  • Peer Reviewed / Open Access
• [Journal Article] Human papillomavirus status of head and neck cancer as determined in cytologic specimens using the hybrid-capture 2 assay. (2014)
  • Author(s): Smith DF, Maleki Z, Coughlan D, Gooi Z, Akpeng B, Ogawa T, Bishop JA, Frick KD, Agrawal N, Gourin CG, Ha PK, Koch WM, Richmon JD, Westra WH, Pai SI
  • Journal Title: Oral Oncology Volume: 50(6) Pages: 600-604
  • Peer Reviewed / Open Access
• [Journal Article] Cold atmospheric plasma treatment selectively targets head and neck squamous cell carcinoma cells. (2014)
  • Author(s): Guerrero-Preston R, Ogawa T, Uemura M, Shumulinsky G, Valle BL, Pirini F, Ravi R, Sidransky D, Keidar M, Trink B
  • Journal Title: International Journal of Molecular Medicine Volume: 34(4) Pages: 941-946
  • Peer Reviewed / Open Access
• [Journal Article] Highlights from the latest articles in epigenomics. (2014)
  • Author(s): Fukushige S, Horii A
  • Journal Title: Epigenomics Volume: 6(2) Issue: 2 Pages: 171-173
  • DOI: 10.2217/epi.14.2
  • Peer Reviewed
• [Journal Article] S100A4 is frequently overexpressed in lung cancer cells and promotes cell growth and cell motility. (2014)
  • Author(s): Chen N, Sato D, Saiki Y, Sunamura M, Fukushige S, Horii A
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 447(3) Pages: 459-464
  • Peer Reviewed
• [Journal Article] Molecular pathology of pancreatic cancer. (2014)
  • Author(s): Saiki Y, Horii A.
  • Journal Title: Pathol Int Volume: 64 Issue: 1 Pages: 10-19
  • DOI: 10.1111/pin.12114
  • Peer Reviewed
• [Journal Article] Human papillomavirus-related carcinoma with adenoid cystic-like features: a peculiar variant of head and neck cancer restricted to the sinonasal tract. (2013)
  • Author(s): Bishop JA, Ogawa T, Stelow EB, Moskaluk CA, Koch WM, Pai SI, Westra WH.
  • Journal Title: Am J Surg Pathol. Volume: 37(6) Issue: 6 Pages: 836-44
  • DOI: 10.1097/pas.0b013e31827b1cd6
  • Peer Reviewed
• [Journal Article] Association between septal deviation and sinonasal papilloma (2013)
  • Author(s): Nomura K, Ogawa T, Sugawara M, Honkura Y, Oshima H, Arakawa K, Oshima T, Katori Y
  • Journal Title: The Tohoku journal of experimental medicine Volume: 231(4) Pages: 315-319
  • NAID: 130004822390
  • Peer Reviewed
• [Journal Article] The expression of S100A4 in human pancreatic cancer is associated with invasion (2013)
  • Author(s): Tsukamoto N, et al
  • Journal Title: Pancreas Volume: 42(6) Issue: 6 Pages: 1027-33
  • DOI: 10.1097/mpa.0b013e31828804e7
  • Peer Reviewed
• [Journal Article] Suppressed expression of NDRG2 correlates with poor prognosis in pancreatic cancer (2013)
  • Author(s): Yamamura A, et al
  • Journal Title: Biochem Biophys Res Commun Volume: 441(1) Issue: 1 Pages: 102-7
  • DOI: 10.1016/j.bbrc.2013.10.010
  • Peer Reviewed
• [Presentation] The role of Human papilloma virus type 16 associated with malignant transformation of sinonasal squamous cell carcinoma (2016)
  • Author(s): Takenori Ogawa,Eiichi Ishida,Kenjiro Higashi,Yuriko Saiki,Shun Sagai,Ayako Nakanome,Akira Okoshi,Masayuki Shirakura,Yukio Katori,Akira Horii
  • Organizer: World Skull Base 2016
  • Place of Presentation: Osaka, Osaka international convention center
  • Year and Date: 2016-06-14
  • Int'l Joint Research
• [Presentation] 鼻副鼻腔乳頭腫由来癌の臨床病理像 (2015)
  • Author(s): 小川武則、野村和弘、荒川一弥、石田英一、中目亜矢子、東賢二郎、石井亮、嵯峨井俊、香取幸夫
  • Organizer: 第27回日本頭蓋底外科学会
  • Place of Presentation: 名古屋、名古屋国際会議場
  • Year and Date: 2015-07-09