| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
In order to develop a new treatment of vocal fold scar, we focused on the smad3 gene, which are known to contribute the scar formation. We planned to inhibit smad3 expression by using smad3 siRNA. Preliminary, we analyzed the pathological feature of ferret vocal fold scar model. The result showed that reduction of the area of lamina propria, increased collagen fiber, and decreased hyaluronic acid at scar side compared with untreated side. Immunostaining showed that collagen 1, collagen3 and fibronectin positive area were significantly increased (p<0.05) in scar side compared with untreated side. These results are similar to the reports of other animal models. We are going to start study using siRNA based on these results.
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