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Development of new treatment for vocal fold scar by inhibiting Smad3 pathway

Research Project

Project/Area Number 25462687
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Otorhinolaryngology
Research InstitutionKumamoto University

Principal Investigator

Masuda Masako  熊本大学, 医学部附属病院, 非常勤診療医師 (70346998)

Co-Investigator(Renkei-kenkyūsha) KUMAI Yoshihiko  熊本大学, 大学院生命科学研究部(医) 耳鼻咽喉科 頭頸部外科, 助教 (00555774)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords声帯瘢痕 / Smad3 / コラーゲン線維 / ヒアルロン酸 / 瘢痕声帯 / コラーゲン1 / コラーゲン3 / si-RNA導入 / フェレット / 遺伝子治療
Outline of Final Research Achievements

In order to develop a new treatment of vocal fold scar, we focused on the smad3 gene, which are known to contribute the scar formation. We planned to inhibit smad3 expression by using smad3 siRNA. Preliminary, we analyzed the pathological feature of ferret vocal fold scar model. The result showed that reduction of the area of lamina propria, increased collagen fiber, and decreased hyaluronic acid at scar side compared with untreated side. Immunostaining showed that collagen 1, collagen3 and fibronectin positive area were significantly increased (p<0.05) in scar side compared with untreated side. These results are similar to the reports of other animal models. We are going to start study using siRNA based on these results.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2014

All Presentation (4 results)

  • [Presentation] Establishing ferret vocal fold scar model2014

    • Author(s)
      Kodama H, Kumai Y,Yumoto E
    • Organizer
      The 9th East Asian Conference on Phonosurgery
    • Place of Presentation
      台湾 International Convention Center
    • Year and Date
      2014-11-29 – 2014-11-30
    • Related Report
      2014 Research-status Report
  • [Presentation] Modulation of MyoD-Positive Muscle Satellite Cells and Myocytes in Rat Thyroarytenoid Muscle Following Recurrent Laryngeal Nerve Regeneration2014

    • Author(s)
      Kumai Y, Kodama H,Nishimoto K,Yumoto E
    • Organizer
      Strategic Directions in Laryngeal Tissue Regeneration
    • Place of Presentation
      (マディソン)米国 Fluno Center for Executive Education
    • Year and Date
      2014-07-10
    • Related Report
      2014 Research-status Report
  • [Presentation] Airway management of midline fixation of the bilateral vocal folds2014

    • Author(s)
      Masuda M, Yumoto E
    • Organizer
      18th World Congress for Bronchoesophagology(WCBE 2014)
    • Place of Presentation
      京都 国立京都国際会館
    • Year and Date
      2014-04-13 – 2014-04-16
    • Related Report
      2014 Research-status Report
  • [Presentation] Postdeglutive residue in associated unilateral laryngeal paralysis vs recurrent laryngeal paralysis2014

    • Author(s)
      Kumai Y, Samejima Y, Yumoto E
    • Organizer
      18th World Congress for Bronchoesophagology(WCBE 2014)
    • Place of Presentation
      京都 国立京都国際会館
    • Year and Date
      2014-04-13 – 2014-04-16
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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