| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Outline of Final Research Achievements |
Objective: The purpose of this study is to regenerate vocal fold mucosa using cultured oral mucosal cells. Methods: A small specimen of oral mucosa was surgically excised from six canines, and epithelial cells were isolated and cultured. The epithelial cells were co-cultured on collagen gels containing separately cultured fibroblasts. The fabricated tissues were autotransplanted to the mucosa-deficient vocal fold. Seven weeks after the transplantation, the vocal fold vibration and morphological characteristics were observed. Results: Laryngeal stroboscopy revealed that the mucosal waves at the transplanted site were regular. Histological findings showed there were fewer elastic fibers in the lamina propria covered with stratified squamous epithelium. The morphology and function after transplantation were more similar to those of a normal vocal fold. Conclusion: The transplantation of the stratified epithelial cell sheet alone has greater ability to regenerate proper vocal fold mucosa.
|
