Involvement of Sulfated Gycosaminoglycan in the Pathogenesis of Diabetic Retinopathy

• Project/Area Number: 25462701
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Hokkaido University
• Principal Investigator: YOSHIZAWA Chikako 北海道大学, 医学(系)研究科(研究院), 客員研究員 (80533653)
• Co-Investigator(Kenkyū-buntansha): SUGAWARA Kazuyuki 北海道大学, 先端生命科学研究科, 名誉教授 (60154449) ; KANDA Atsuhiro 北海道大学, 大学院医学研究科, 特任講師 (80342707)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 硫酸化グリコサミノグリカン / 糖尿病網膜症 / 糖鎖 / 硫酸化グルコサミノグリカン

Outline of Final Research Achievements

The white blood cells were purified from diabetes-induced mouse model, and analyzed composition of sulfated glycosaminoglycan. However, compared to control mice, significant difference was not observed. Therefore, we comprehensively analyzed sugar chains in the vitreous fluids from diabetic retinopathy patients. As results, total glycan amount including sulfated glycosaminoglycan were significantly elevated in diabetic retinopathy patients than in non-diabetic patients (control group). This suggests that increased glycans may be involved in the pathogenesis of diabetic retinopathy.

Research Products

• [Journal Article] Changes in inner and outer retinal layer thicknesses after vitrectomy for idiopathic macular hole: Implications for visual prognosis (2015)
  • Author(s): Hashimoto Y, Saito W, Fujiya A, Yoshizawa C, Hirooka K, Mori S, Noda K, Ishida S
  • Journal Title: PLoS One Volume: 10 Issue: 8 Pages: e0135925-e0135925
  • DOI: 10.1371/journal.pone.0135925
  • Peer Reviewed / Open Access
• [Journal Article] Alteration of N-glycan profiles in Diabetic Retinopathy (2015)
  • Author(s): Inafuku S., Noda K., Amano M., Murata M., Saito W., Ohashi T., Kanda A., Nishimura S-I., Ishida S.
  • Journal Title: Invest. Ophthalmol. Vis. Sci. Volume: 56 Issue: 9 Pages: 5316-5322
  • DOI: 10.1167/iovs.15-16747
  • Peer Reviewed
• [Journal Article] A comparison of N-glycan profiles in human plasma and vitreous fluid. (2014)
  • Author(s): Inafuku S, Noda K, Amano M, Ohashi, Yoshizawa C, Saito W, Kanda A, Nishimura S, Ishida S.
  • Journal Title: Graefes Arch Clin Exp Ophthalmol. Volume: 252 Issue: 8 Pages: 1235-1243
  • DOI: 10.1007/s00417-014-2671-x
  • Peer Reviewed / Open Access
• [Journal Article] Macular choroidal blood flow velocity decreases with regression of acute central serous chorioretinopathy. (2013)
  • Author(s): Saito M, Saito W, Hashimoto Y, Yoshizawa C, Fujiya A, Noda K, Ishida S.
  • Journal Title: Br J Ophthalmol. Volume: 97 Pages: 775-780
  • Peer Reviewed
• [Journal Article] Photodynamic therapy combined with intravitreal bevacizumab and sub-tenon triamcinolone acetonide injections for age-related macular degeneration. (2013)
  • Author(s): Yoshizawa C, Saito W, Hirose S, Kitamei H, Noda K, Ishida S.
  • Journal Title: Jpn J Ophthalmol. Volume: 57 Pages: 68-73
  • NAID: 10031168357
  • Peer Reviewed
• [Presentation] PDTトリプル療法を行ったポリープ状脈絡膜血管症における治療1年後視力の規定因子 (2015)
  • Author(s): 安藤 亮、齋藤 航、廣岡季里子、鈴木智浩、吉澤史子、齋藤理幸、加瀬 諭、森 祥平、野田航介、石田 晋
  • Organizer: 第54回日本網膜硝子体学会総会、第32回日本眼循環学会合同学会
  • Place of Presentation: 東京国際フォーラム（東京都・千代田区）
  • Year and Date: 2015-12-04
• [Presentation] アフリベルセプト併用PDTトリプル療法1年後の治療成績 (2015)
  • Author(s): 鈴木智浩、齋藤 航、森 祥平、齋藤理幸、安藤 亮、加瀬 諭、廣岡季里子、吉澤史子、野田航介、石田 晋
  • Organizer: 第54回日本網膜硝子体学会総会、第32回日本眼循環学会合同学会
  • Place of Presentation: 東京国際フォーラム（東京都・千代田区）
  • Year and Date: 2015-12-04
• [Presentation] 2型脈絡膜新生血管を伴った加齢黄斑変性に対するPDTトリプル療法の治療成績 (2015)
  • Author(s): 森 祥平、齋藤 航、齋藤理幸、安藤 亮、廣岡季里子、吉澤史子、野田航介、石田 晋
  • Organizer: 第54回日本網膜硝子体学会総会、第32回日本眼循環学会合同学会
  • Place of Presentation: 東京国際フォーラム（東京都・千代田区）
  • Year and Date: 2015-12-04
• [Presentation] 糖尿病網膜症の硝子体および血漿におけるN型糖鎖の網羅的解析 (2014)
  • Author(s): 稲福沙織、野田航介、天野麻穂、大橋哲、吉澤史子、齋藤 航、村田美幸、神田敦宏、西村紳一郎、石田 晋.
  • Organizer: 第68回日本日本臨床眼科学会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2014-11-13 – 2014-11-16