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Functional analysis of vitreous cells in intraocular proliferative disease towards a new treatment strategy

Research Project

Project/Area Number 25462704
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ophthalmology
Research InstitutionYamagata University

Principal Investigator

NISHITSUKA KOICHI  山形大学, 医学部, 講師 (60422169)

Co-Investigator(Renkei-kenkyūsha) YAMASHITA Hidetoshi  山形大学, 医学部, 教授 (90158163)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords硝子体細胞 / 増殖糖尿病網膜症 / 増殖硝子体網膜症 / 硝子体手術 / 樹状細胞 / ヒアルロナン / 網膜硝子体疾患
Outline of Final Research Achievements

To investigate the the role of hyalocytes on pathogesis of the intra ocular proliferative diseases, we performed to construct the co-culture system with porcine vitreous derived cells, human vitreous derived cells and the other several kinds of cells include grial cell, endothelial cell and dendritic cell. Hyalocytes effect cell-cell interaction to those cells. It may be concerened that hyalocytes act to the pathogenesis via complicated cell-cell interactions.
Liquid based cytology could be applied to clinical vitreous analysi. There were several kinds of cells include dendritic cell in vitreous. Liquid based cytology could also enable clinical diagnosis (malignant lymphoma and vitreous amyloidosis) .

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (18 results)

All 2015 2014 2013

All Journal Article (6 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (12 results)

  • [Journal Article] A survey of vitreous cell components performed using liquid-based cytology2015

    • Author(s)
      Mari Narumi, Koichi Nishitsuka,Mitsunori Yamakawa, Hidetoshi Yamashita
    • Journal Title

      Acta Ophthalmologica

      Volume: 印刷中 Issue: 5

    • DOI

      10.1111/aos.12623

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Minimally Invasive Scleral Fixation of an IOL Using a Suture Thread Inserter Combined With 25-Gauge Vitrectomy.2014

    • Author(s)
      Koichi Nishitsuka, Hidetoshi Yamashita
    • Journal Title

      Ophthalmic Surg Lasers Imaging Retina.

      Volume: 45 Issue: 4 Pages: 318-322

    • DOI

      10.3928/23258160-20140625-02

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 【網膜血管疾患アップデート】 糖尿病網膜症の手術治療の進歩2014

    • Author(s)
      西塚弘一
    • Journal Title

      あたらしい眼科

      Volume: 31 Pages: 1089-1095

    • Related Report
      2014 Research-status Report
  • [Journal Article] 【血管内皮と糖尿病とその合併症】 血管内皮障害と糖尿病網膜症2014

    • Author(s)
      西塚弘一、山下英俊
    • Journal Title

      内分泌・糖尿病・代謝内科

      Volume: 39 Pages: 472-479

    • Related Report
      2014 Research-status Report
  • [Journal Article] 【糖尿病をめぐる話題と治療の現況】 合併症対策 糖尿病網膜症2013

    • Author(s)
      西塚弘一、山下英俊
    • Journal Title

      医学と薬学

      Volume: 71 Pages: 67-71

    • Related Report
      2013 Research-status Report
  • [Journal Article] 高齢者の糖尿病】 高齢者糖尿病患者の合併症の管理 細小血管合併症 糖尿病網膜症・黄斑症2013

    • Author(s)
      西塚弘一、山下英俊
    • Journal Title

      日本臨床

      Volume: 71 Pages: 2005-2009

    • Related Report
      2013 Research-status Report
  • [Presentation] 周辺部硝子体処理適正化のための術中OCTによる周辺部硝子体の定量の試み2015

    • Author(s)
      西塚 弘一,西 勝弘,武田 祐介,阿部 さち,桐井 枝里子,難波 広幸,菅野 彰,赤羽 梢,村上 敬憲,松下高幸,金子 優,山下 英俊
    • Organizer
      第54回日本網膜硝子体学会総会
    • Place of Presentation
      東京国際フォーラム(東京都港区)
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] 硝子体細胞診の臨床的意義2015

    • Author(s)
      西塚 弘一,成味 真梨,菅野 彰,金子 優,鈴木 俊紀,山川 光徳,山下 英俊,
    • Organizer
      第69回日本臨床眼科学会総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 糖尿病網膜症の病態に迫る 糖尿病網膜症における硝子体細胞と細胞外基質の役割2015

    • Author(s)
      西塚弘一
    • Organizer
      第119回日本眼科学会総会
    • Place of Presentation
      札幌市教育文化会館(北海道札幌市)
    • Year and Date
      2015-04-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 空気タンポナーデを行った裂孔原性網膜剥離体する硝子体手術2014

    • Author(s)
      西塚弘一,西勝弘,武田祐介,阿部さち,桐井枝里子,難波広幸,三浦瞳,菅野彰,成味真梨,赤羽梢,村上敬憲,金子優,山下英俊
    • Organizer
      第53回網膜硝子体学会総会
    • Place of Presentation
      大阪、大阪国際会議場
    • Year and Date
      2014-11-28 – 2014-11-30
    • Related Report
      2014 Research-status Report
  • [Presentation] 25Gトロカール創口に縫合糸結び目を埋没した小切開硝子体手術・眼内レンズ縫着術2014

    • Author(s)
      西塚弘一、山下英俊
    • Organizer
      第68回日本臨床眼科学会
    • Place of Presentation
      神戸、ポートピアホテル、神戸国際展示場
    • Year and Date
      2014-11-13 – 2014-11-16
    • Related Report
      2014 Research-status Report
  • [Presentation] A modified scleral IOL fixation technique using a suture thread inserter combined with 25-gauge vitrectomy,2014

    • Author(s)
      4)Koichi Nishitsuka, Hidetoshi Yamashita:
    • Organizer
      27TH INTERNATIONAL CONGRESS OF GERMAN OPHTHALMIC SURGEONS
    • Place of Presentation
      ドイツ、ニュルンベルグ、Nurmberg convention center
    • Year and Date
      2014-05-15 – 2014-05-17
    • Related Report
      2014 Research-status Report
  • [Presentation] Effects of human recombinant hyaluronidase in porcine vitreous2014

    • Author(s)
      Koichi Nishitsuka, Mari Narumi, Hidetoshi Yamashita
    • Organizer
      87th The Association for Research in Vision and Ophthalmology Annual meeting
    • Place of Presentation
      アメリカ、オーランド、Orange County Convention Center
    • Year and Date
      2014-05-04 – 2014-05-08
    • Related Report
      2014 Research-status Report
  • [Presentation] Ophthalmic endoscope-assisted early vitrectomy for endophthalmitis with hazy media2014

    • Author(s)
      Koichi Nishitsuka, Hidetoshi Yamashita
    • Organizer
      World Ophthalmology Congress 2014
    • Place of Presentation
      東京、東京フォーラム
    • Year and Date
      2014-04-02 – 2014-04-06
    • Related Report
      2014 Research-status Report
  • [Presentation] Analysis of vitreous hyaluronan status in vitreoretinal diseases.2013

    • Author(s)
      Koichi Nishitsuka, Mari Narumi,Yoshiko Kashiwagi, Takuma Shibata, Jin Gong, Hidemitsu Furukawa, Hidetoshi Yamashita
    • Organizer
      86th The Association for Research in Vision and Ophthalmology Annual meeting
    • Place of Presentation
      アメリカ、シアトル,Washington State Convention Center
    • Related Report
      2013 Research-status Report
  • [Presentation] Minimally invasive scleral fixation of the intraocular lens using suture thread inserter combined with 25-Gauge vitrectomy2013

    • Author(s)
      Koichi Nishitsuka, Hidetoshi Yamashita
    • Organizer
      26TH INTERNATIONAL CONGRESS OF GERMAN OPHTHALMIC SURGEONS
    • Place of Presentation
      ドイツ、ニュルンベルグ,nurmberg convention center
    • Related Report
      2013 Research-status Report
  • [Presentation] Treatment outcome of scleral fixation of the intraocular lens using a suture thread inserter2013

    • Author(s)
      Koichi Nishitsuka , Hidetoshi Yamashita
    • Organizer
      8th APVRS Congress & 52nd Annual Meeting of Japanese Retina and Vitreous Society.
    • Place of Presentation
      名古屋,名古屋国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] 網膜硝子体疾患における硝子体内ヒアルロナン濃度の検討2013

    • Author(s)
      西塚 弘一、成味 真梨、柏木 佳子、武田 祐介、阿部 さち、 望月 典子、山下 英俊
    • Organizer
      第117回日本眼科学会総会
    • Place of Presentation
      東京、東京フォーラム
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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