| Project/Area Number |
25462708
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
Ohishi Kentaro 浜松医科大学, 光尖端医学教育研究センター, 助教 (80345826)
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| Co-Investigator(Kenkyū-buntansha) |
大坪 正史 浜松医科大学, 光尖端医学教育研究センター, 助教 (10327653)
尾花 明 浜松医科大学, 光尖端医学教育研究センター, 客員教授 (40194625)
堀田 喜裕 浜松医科大学, 医学部, 教授 (90173608)
蓑島 伸生 浜松医科大学, 光尖端医学教育研究センター, 教授 (90181966)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 網膜光障害 / ラット / 戻し交配 / 系統差 / 責任遺伝子 / エキソーム解析 / 加齢黄斑変性 / 次世代シーケンサー / 網膜変性 / 感受性遺伝子 / 光 / 連続戻し交配 |
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| Outline of Final Research Achievements |
PURPOSE: To identify genes for photic injury susceptibility of rat retina, eventually to elucidate an etiology for age-related macular degeneration (AMD). METHODS: A retinal degeneration size showing the susceptibility was evaluated on pathological specimens. A targeted exome analysis was performed with MiSeq system (illumina, Inc.). RESULTS: We performed the targeted exome analysis for 3.8-Mb Rpi1 region among resistant (LEW) and susceptible (WKY and F344) strains and found 3 loci with non-synonymous base changes in 2 genes. In parallel, obtained BC9 offspring had a 284-kb WKY genome region as a sole WKY component, and in which only 1 locus in 1 gene (tentatively gene R) was narrowed down. CONCLUSION: We consider that the gene R is Rpi1 itself. The gene R has never been reported as the susceptibility gene of AMD. We are now extensively making efforts to give more direct evidence for the gene R as the causal gene of rat retinal photic injury and possible relationship with AMD.
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