Investigation of a new mechanism of age-related macular degeneration using a rat model of retinal photic injury: targeted exome analysis for a responsible genomic region
| Project/Area Number |
25462708
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
Ohishi Kentaro 浜松医科大学, 光尖端医学教育研究センター, 助教 (80345826)
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| Co-Investigator(Kenkyū-buntansha) |
大坪 正史 浜松医科大学, 光尖端医学教育研究センター, 助教 (10327653)
尾花 明 浜松医科大学, 光尖端医学教育研究センター, 客員教授 (40194625)
堀田 喜裕 浜松医科大学, 医学部, 教授 (90173608)
蓑島 伸生 浜松医科大学, 光尖端医学教育研究センター, 教授 (90181966)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 網膜光障害 / ラット / 戻し交配 / 系統差 / 責任遺伝子 / エキソーム解析 / 加齢黄斑変性 / 次世代シーケンサー / 網膜変性 / 感受性遺伝子 / 光 / 連続戻し交配 |
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| Outline of Final Research Achievements |
PURPOSE: To identify genes for photic injury susceptibility of rat retina, eventually to elucidate an etiology for age-related macular degeneration (AMD). METHODS: A retinal degeneration size showing the susceptibility was evaluated on pathological specimens. A targeted exome analysis was performed with MiSeq system (illumina, Inc.). RESULTS: We performed the targeted exome analysis for 3.8-Mb Rpi1 region among resistant (LEW) and susceptible (WKY and F344) strains and found 3 loci with non-synonymous base changes in 2 genes. In parallel, obtained BC9 offspring had a 284-kb WKY genome region as a sole WKY component, and in which only 1 locus in 1 gene (tentatively gene R) was narrowed down. CONCLUSION: We consider that the gene R is Rpi1 itself. The gene R has never been reported as the susceptibility gene of AMD. We are now extensively making efforts to give more direct evidence for the gene R as the causal gene of rat retinal photic injury and possible relationship with AMD.
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Report
(5 results)
Research Products
(13 results)
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[Presentation] Mapping Susceptibility Loci to Rat Retinal Photic Injury2013
Author(s)
Ohishi, K., Hosono, K., Obana, A., Hotta, Y., Hiramitsu, T., Minoshima, T.
Organizer
1st International Symposium on Blue Light Society
Place of Presentation
東京
Related Report
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