Function of retinal pigment epithelium in retinitis pigmentosa

• Project/Area Number: 25462709
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Nagoya University
• Principal Investigator: Ueno Shinji 名古屋大学, 医学部附属病院, 病院講師 (80528670)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 網膜色素変性 / 網膜電図 / 網膜変性動物モデル / 網膜色素上皮 / 遺伝子改変ウサギ / 網膜変性ウサギ / 網膜変性 / ウサギ / 局所網膜電図

Outline of Final Research Achievements

In this study we used rhodopsin P347L transgenic (Tg) rabbits which are models of retinitis pigmentosa. We confirmed the full-field ERG of these Tg rabbit became non-recordable between 24 and 36 months. We performed subretinal injection of PBS to these old Tg rabbits and created retinal detachment artificially. The subretinal fluied was resolved within 24 hours after injections and there were no difference in the recovery time of retinal detachment between Tg and control rabbits. Structures of RPE evaluated by transmission microscope showed no specific abnormalities compared to those of wild type rabbits. Our results indicated even after photoreceptor death the function of RPE were still preserved normally.

Research Products

• [Journal Article] Temporal Properties of Cone ERGs of Pikachurin Null Mutant Mouse. (2016)
  • Author(s): 1.Kominami T, Ueno S, Nakanishi A, Kominami A, Kondo M, Furukawa T, Terasaki H.
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 57 Issue: 3 Pages: 1264-1269
  • DOI: 10.1167/iovs.15-17708
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Pathologic Changes of Cone Photoreceptors in Eyes With Occult Macular Dystrophy. (2015)
  • Author(s): Nakanishi A, Ueno S, Kawano K, Ito Y, Kominami T, Yasuda S, Kondo M, Tsunoda K, Iwata T, Terasaki H.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: Nov;56(12) Issue: 12 Pages: 7243-9
  • DOI: 10.1167/iovs.15-16742
  • Peer Reviewed / Open Access
• [Journal Article] Focal cone ERGs of rhodopsin Pro347Leu transgenic rabbits. (2013)
  • Author(s): Ueno S, Koyasu T, Kominami T, Sakai T, Kondo M, Yasuda S, Terasaki H
  • Journal Title: Vision Res. Volume: 91 Pages: 118-123
  • DOI: 10.1016/j.visres.2013.08.006
  • Peer Reviewed
• [Presentation] Correlation between focal macular electroretinograms and length of interdigitation line of photoreceptor after succesful surgery for fovea off rhegmatogenous retinal detachment (2015)
  • Author(s): 上野 真治
  • Organizer: 第54回日本網膜硝子体学会
  • Place of Presentation: 東京都千代田区 東京国際フォーラム
  • Year and Date: 2015-12-05
• [Presentation] ロドプシンP347L遺伝子改変ウサギの網膜機能の推移、組織学的変化 (2015)
  • Author(s): 小南太郎 上野真治 廣田里香 小南梓 中西絢美 長屋匡俊 近藤峰生 寺崎浩子
  • Organizer: 第119回日本眼科学会総会
  • Place of Presentation: ロイトン札幌ホテル（北海道札幌市）
  • Year and Date: 2015-04-16 – 2015-04-19
• [Presentation] 双極細胞変性をきたす腫瘍随伴網膜症 (2014)
  • Author(s): 上野 真治
  • Organizer: 熊本眼科フォーラム
  • Place of Presentation: 熊本医師会館 （熊本市）
  • Invited
• [Presentation] ON型双極細胞の機能が回復した腫瘍随伴症の一例 (2013)
  • Author(s): 上野 真治
  • Organizer: 第61回日本臨床視覚電気生理学会
  • Place of Presentation: 千里ライフサイエンスセンター(大阪市）