| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
We investigated the expression of Palladin in corneal stroma. Palladin was expressed in the scar-forimng diseased corneas and stromal wound healing process, and it co-localised with alpha-smooth muscle actin (alpha-SMA). TGF beta stimulation on human corneal fibroblasts (CFb) increased 140 kDa Palladin in dose-dependent manner. The inhibition of TGF beta signaling decreased ecpression of palladin. Palladin RNA interefered Cfb decreased the expression of alpha-SMA by TGF beta stimualtion. Also, Gel contraction assay for Palladin RNA interefered CFb revealed the decreased shrinkage of collagen gel by the stimulation of TGF beta. Our data indicated that palladin was closely related to the scar formation, indicating that palladin may be a candidate molecule to regulate corneal scaring.
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