Palladin relates scar formation in the corneal stroma.
Project/Area Number |
25462716
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Ophthalmology
|
Research Institution | Yamaguchi University |
Principal Investigator |
MORISHIGE Naoyuki 山口大学, 医学(系)研究科(研究院), 講師 (40346565)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | Palladin / 角膜実質 / 瘢痕形成 / α平滑筋アクチン / TGFβ / パラディン / 角膜 / 実質 / 創傷治癒 / 筋線維芽細胞 |
Outline of Final Research Achievements |
We investigated the expression of Palladin in corneal stroma. Palladin was expressed in the scar-forimng diseased corneas and stromal wound healing process, and it co-localised with alpha-smooth muscle actin (alpha-SMA). TGF beta stimulation on human corneal fibroblasts (CFb) increased 140 kDa Palladin in dose-dependent manner. The inhibition of TGF beta signaling decreased ecpression of palladin. Palladin RNA interefered Cfb decreased the expression of alpha-SMA by TGF beta stimualtion. Also, Gel contraction assay for Palladin RNA interefered CFb revealed the decreased shrinkage of collagen gel by the stimulation of TGF beta. Our data indicated that palladin was closely related to the scar formation, indicating that palladin may be a candidate molecule to regulate corneal scaring.
|
Report
(4 results)
Research Products
(23 results)