Role of Rho kinase on epithelial mesenchymal transformation of lens epithelial cells induced by TGF-b

• Project/Area Number: 25462732
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Iwate Medical University
• Principal Investigator: Kurosaka Daijiro 岩手医科大学, 医学部, 教授 (20215099)
• Co-Investigator(Kenkyū-buntansha): HASHIDUME Kouhei 岩手医科大学, 眼科学, 助教 (50407095) ; TAMADA Kunifusa 岩手医科大学, 眼科学, 助教 (20583610)
• Research Collaborator: IMAIZUMI Toshiyasu
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: Rock inhibitor / 上皮間葉系転換 / 水晶体上皮細胞 / 前嚢下白内障 / TGF-β / Rhoキナーゼ / 上皮間葉系移行 / Y-27632 / ROCK阻害剤

Outline of Final Research Achievements

Epithelial mesenchymal transformation (EMT) of lens epithelial cells (LECs) brings about anterior subcapsular cataract (ASC). We have reported that TGF-βplays an important role and Rho is related to its intracellar signal transduction. The effect of Rock inhibitor (Y-27632) on EMT was evaluated.
Y-27632 inhibited production of type I collagen and alfa-smooth muscle actin, and cellar contactibility of LECs in vitro and formation of ASC in vivo. These findings suggested that Rock inhibitor may be a candidate of protective drug of ASC.

Research Products

• [Presentation] 豚水晶体上皮細胞遊走に対するTGF-β2とMRTF阻害剤の影響 (2016)
  • Author(s): 今泉利康、奥野孟、小笠原聡、木澤純也、黒坂大次郎
  • Organizer: 第120回日本眼科学会総会
  • Place of Presentation: 仙台、仙台国際センター
  • Year and Date: 2016-04-07
• [Presentation] 豚水晶体上皮細胞遊走に対するTGF-βとRhoキナーゼ阻害剤の影響 (2015)
  • Author(s): 今泉利康
  • Organizer: 第54回日本白内障学会総会
  • Place of Presentation: 名古屋、ミッドランドスクエア オフィスタワー５F
  • Year and Date: 2015-09-18
• [Presentation] TGF-β2による水晶体上皮細胞のコラーゲンゲル収縮へのMRTF阻害剤の影響 (2015)
  • Author(s): 今泉 利康, 前田 可奈子, 小笠原 聡, 木澤 純也, 黒坂 大次郎
  • Organizer: 第119回日本眼科学会総会
  • Place of Presentation: 札幌、ロイトン札幌
  • Year and Date: 2015-04-16
• [Presentation] TGF-βによる水晶体上皮細胞の収縮能亢進に対するRhoキナーゼ阻害剤の影響 (2014)
  • Author(s): 今泉利康、前田可奈子、小笠原聡、黒坂大次郎
  • Organizer: 第５３回日本白内障学会
  • Place of Presentation: TKPガーデンシティ品川(東京・品川)
  • Year and Date: 2014-09-27