Development of New Therapeutic Strategies for Animal Models of Retinal Degeneration by chitin and chitosan.

• Project/Area Number: 25462740
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Kansai Medical University
• Principal Investigator: YOSHIZAWA Katsuhiko 関西医科大学, 医学部, 講師 (70548396)
• Co-Investigator(Kenkyū-buntansha): TSUBURA Airo 関西医科大学, 医学部, 教授 (90098137) ; YURI Takashi 関西医科大学, 医学部, 講師 (50330212)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 網膜色素変性症 / 視細胞 / 緑茶抽出物 / クルクミン / 酸化ストレス / ＭＮＵ / 動物モデル / 併用効果 / エピガロカテキンガレート / MNU

Outline of Final Research Achievements

The purpose of the present study was to evaluate the efficacy of curcumin (CUR) and green tea extracts (GTE) as antioxidants against MNU-induced photoreceptor apoptosis. Furthermore, combinational effects were evaluated. Seven-week-old female Sprague-Dawley rats received a single intraperitoneal administration of 40 mg/kg MNU. Intraperitoneal administration of 200mg/kg CUR or oral administration of 250mg/kg GTE started 3 days prior to MNU exposure, and the administration was continued once daily for 10 days. Both CUR and GTE inhibited MNU-induced photoreceptor cell apoptosis by suppressing oxidative stress as seen by oxidative makers. These findings indicate that CUR and GTE may help to suppress the onset and progression of human RP. However, we could not confirm additive action and/or synergistic effect by CUR and GTE.

Research Products

• [Journal Article] Mead acid supplementation does not rescue rats from cataract and retinal degeneration induced by N-methyl-N-nitrosourea. (2015)
  • Author(s): Emoto Y, Yoshizawa K, Hamazaki K, Kinoshita Y, Yuki M, Yuri T, Kawashima H, Tsubura A.
  • Journal Title: Journal of Toxicologic Pathology Volume: 28 Pages: 11-20
  • NAID: 130004690896
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Green tea extract suppresses N-methyl-N-nitrosourea-induced photoreceptor apopptosis in Sprague-Dawley rats. (2014)
  • Author(s): Emoto Y, Yoshizawa K, Kinoshita Y, Yuri T, Yuki M, Sayama K, Shikata N, Tsubura A.
  • Journal Title: Graefes Arch Clin Exp Ophthalmol Volume: 252 Issue: 9 Pages: 1377-1384
  • DOI: 10.1007/s00417-014-2702-7
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Green Tea Extract-induced Acute Hepatotoxicity in Rats (2014)
  • Author(s): Emoto Y, Yoshizawa K, Kinoshita Y, Yuki M, Yuri T, Yoshikawa Y, Sayama K, Tsubura A.
  • Journal Title: Journal of Toxicologic Pathology Volume: 27 Issue: 3+4 Pages: 163-174
  • DOI: 10.1293/tox.2014-0007
  • NAID: 130004713011
  • ISSN: 0914-9198, 1881-915X
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Similarity of GATA-3 Expression between Rat and Human Mammary Glands (2014)
  • Author(s): Kinoshita Y, Yoshizawa K, Emoto Y, Yuki M, Yuri T, Shikata N, Tsubura A.
  • Journal Title: Journal of Toxicologic Pathology Volume: 27 Issue: 2 Pages: 159-162
  • DOI: 10.1293/tox.2014-0008
  • NAID: 130004677739
  • ISSN: 0914-9198, 1881-915X
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Curcumin suppresses N-methyl-N-nitrosourea-induced photoreceptor apoptosis in Sprague-Dawley rats. (2013)
  • Author(s): Emoto Y, Yoshizawa K, Uehara N, Kinoshita Y, Yuri T, Shikata N, Tsubura A.
  • Journal Title: In Vivo Volume: 27(5 Pages: 583-590
  • Peer Reviewed
• [Journal Article] N-methyl-N-nitrosourea-induced cerebellar hypoplasia in rats: effect of arachidonic acid supplementation during the gestational, lactational and post-weaning periods. (2013)
  • Author(s): Yoshizawa K, Emoto Y, Kinoshita Y, Yuri T, Tsubura A.
  • Journal Title: Experimental and Therapeutic Medicine Volume: 6 Issue: 3 Pages: 627-634
  • DOI: 10.3892/etm.2013.1219
  • Peer Reviewed
• [Presentation] MNU誘発白内障ならびに網膜変性症モデルに対するミード酸補給の効果. (2015)
  • Author(s): 榎本祐子, 義澤克彦, 浜崎 景, 木下勇一, 結城美智子, 圦 貴司, 河島 洋, 螺良愛郎
  • Organizer: 第104回日本病理学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-04-30
• [Presentation] MNU誘発ラット網膜色素変性症モデルに対する緑茶抽出物の有用性. (2014)
  • Author(s): 榎本祐子, 義澤克彦, 結城美智子, 木下勇一, 圦 貴司, 四方伸明, 螺良愛郎.
  • Organizer: 第103回日本病理学会
  • Place of Presentation: 広島（広島国際会議場）
  • Year and Date: 2014-04-25
• [Presentation] 緑茶抽出物によるラット肝臓毒性. (2014)
  • Author(s): 義澤克彦, 榎本祐子, 木下勇一, 圦 貴司, 結城美智子, 吉川 豊, 茶山和敏, 螺良愛郎.
  • Organizer: 第103回日本病理学会
  • Place of Presentation: 広島（広島国際会議場）
  • Year and Date: 2014-04-25
• [Presentation] 緑茶抽出物誘発のラット肝臓毒性の特徴.
  • Author(s): 榎本祐子, 義澤克彦, 木下勇一, 圦 貴司, 吉川 豊, 茶山和敏, 螺良愛郎.
  • Organizer: 第30回日本毒性病理学会
  • Place of Presentation: 徳島（あわぎんホール）
• [Presentation] MNU誘発ラット網膜色素変性症モデルに対するクルクミンの有用性.
  • Author(s): 榎本祐子, 義澤克彦, 木村彩子, 木下勇一, 圦 貴司, 四方伸明, 螺良愛郎.
  • Organizer: 第102回日本病理学会
  • Place of Presentation: 札幌（ロイトン札幌）
• [Book] Recent Advances in Eye Research. N-Methyl-N-Nitrosourea-Induced Retinal Degeneration in Rats: A Reliable Animal Model of Retinitis Pigmentosa for the Development of New Therapeutic Strategies. (2015)
  • Author(s): Yoshizawa K, Emoto Y, Tsubura A.
  • Total Pages: 197
  • Publisher: Nova Science