| Project/Area Number |
25462747
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Iwate University |
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Principal Investigator |
SUGANO ERIKO 岩手大学, 工学部, 准教授 (70375210)
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| Co-Investigator(Kenkyū-buntansha) |
ISAGO Hitomi 東北大学, 大学病院, 助手 (30400451)
村山 奈美枝 東北大学, 大学病院, その他 (60597516)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | オプトジェネティクス / 再生医学 / 生理学 / 脳・神経 / 網膜色素変性症 / 視細胞変性 / チャネルロドプシン / アデノ随伴ウイルスベクター / 眼科学 / オプトジェネェティクス / 網膜神経節細胞 |
|---|
| Outline of Final Research Achievements |
Modifierd vorvox derived channelrhodopsin-1 (mVChR1) or halorhodopisin(NpHR) gene was inserted into adeno-associated virus vector (AAV), and capsid modified AAV2 was most efficient vector for retinal ganglion cells. However, NpHR was low expression in mammalian cells by AAV gene transfer. We modified the transmembrane signal of NpHR {m(em)-NpHR}, however, there was not sufficient to improve the expression and function compared to that of ChR2 protein in vivo and vitro. We also studied about the 3-dimentional structural analysis of protein on data base, these analyze indicated that there might be a problem in the pathway of the ion and result in low light sensitivity.
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