| Project/Area Number |
25462751
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Chiba University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | RGC neuropathy / 糖尿病網膜症 / 神経保護 / 神経突起再生 / neurotorphin-4 / NF-kB / SP1 / 網膜3次元培養 / neurotrophin-4 / 神経血管単位 / TUDCA / 終末糖化産物 |
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| Outline of Final Research Achievements |
Neuronal abnormalities of diabetic retinopathy are irreversible changes and accumulated below the surface. These changes are directly related to permanent vision loss. RGC neuropathy is a progressive optic nerve neuropathy with RGC death and axonal degeneration in diabetic patients. Thus, neuroprotective and regenerative therapies that inhibit the progression of RGC loss and axonal degeneration are greatly required. For these purpose, we used three-dimensional collagen gel culture of rat retinas. Retinal explants were incubated with AGEs simultaneously supplemented with NT-4, HGF, GDNF, and TUDCA. In retinas supplemented with NT-4, the number of regenerating neurites were the most among all groups. In addition, immunopositivities of NF-kB, SP1, JNK and p38 were increased in retinas incubated with AGEs, and decreased in retinas with AGEs supplemented with neurotrophic factors. These results indicate that NF-kB, JNK and p38 are proapoptotic factors in diabetic retinal neurons.
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