| Project/Area Number |
25462756
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Ehime University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Ohashi Yuichi 愛媛大学, 大学院医学系研究科, 教授 (00116005)
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| Co-Investigator(Renkei-kenkyūsha) |
Takeshi Imamura 愛媛大学, 大学院医学系研究科, 教授 (70264421)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ケラトサイト / 角膜実質 / 幹細胞 / バイオイメージング / 再生医療 / keratocan / keratocyte / bio-imaging / gene targeting |
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| Outline of Final Research Achievements |
Keratocan-IRES2-nls-Cre and Keratocan-IRES2-CreERT2 knock-in mouse lines were newly developed by gene targeting technique using C57BL/6-derived ES cells for keratocyte specific Cre-lox P recombination. These mice were mated with ROSA26mT/mG or ROSA26LacZ to investigate temporal and spatial Cre recombinase expression pattern. In Keratocan-IRES2-nls-Cre/ ROSA26LacZ embryo, Cre recombinase expression was observed in periocular mesenchymal cells. After birth, Cre recombinase expression were observed in keratocytes and hippocampal CA1 pyramidal cells. In keratocytes of Keratocan-IRES2-CreERT2/ROSA26mT/mG mice, Cre-lox P recombination were observed after tamoxifen induction. These mouse lines will become useful model for keratocyte stem cell biology.
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