| Project/Area Number |
25462776
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SUDA HIROKO 熊本大学, 医学部附属病院, 非常勤診療医師 (40632659)
YOKOUCHI YUJI 福島県立医科大学, 医学部, 教授 (60252227)
SAKAMOTO SEISUKE 熊本大学, 医学部附属病院, 非常勤診療医師 (00378689)
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| Research Collaborator |
YOSHII DAIKI 熊本大学, 医学部附属病院, 大学院生
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 胆道閉鎖症 / SOX9遺伝子 / 細胆管反応 / 葛西手術 / 胆汁うったい / ＳＯＸ９遺伝子 / Notch Signal / Sox9遺伝子 / 細胆管増生 / 肝線維化 |
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| Outline of Final Research Achievements |
The mechanism of ductular reaction (DR) seen in liver diseases including biliary atresia (BA) has been still controversial. Notch signaling has been reported to involve in biliary development and differentiation in vivo. The aim was to elucidate the role of SOX9+ biphenotypic cells in DR and the association between Notch signaling and development of DR. Formalin fixed paraffin embedded liver sections from 47 liver specimens with BA were used. Image processing was performed by Image J 1.46 software after immunofluorescence staining. Sustained or progressive cholestasis led to decreasing of SOX9+HepPar1+ ratio and increasing CK19+ ratio, whereas reduction of jaundice led to increasing of SOX9+HepPar1+ ratio and decreasing CK19+ ratio. SOX9+HepPar1+ ratio had significant negative correlation with CK19+ ratio (r = -0.6, p = 0.002). In conclusion, DR in BA is mediated by SOX9+ biphenotypic cells, and Notch signaling is presumably required for development of DR.
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