| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
It is widely accepted that new tumor blood vessels sprout from pre-existing vasculature. However, recent evidence suggests that CD133-positive cancer stem cells (CSCs) in glioblastomas generate tumor endothelial progenitor cells, which further differentiate into tumor endothelial cells. It was investigated the relationship between tumor endothelial cells and CSCs in human hepatoblastoma. Examination of the CD133-positive sites using SEM revealed that they coincided with filopodia and lamellipodia. Digested xenograft tumor fragments were cultured and tumor sphere assay was carried out. The spheres were cultivated using three-dimensional collagen gel culture methods. Some spheres formed CD133-positive capillary-like structures. TEM imaging of these structures confirmed the presence of identifiable lumens. The observations suggest that CD133-positive CSCs in hepatoblastoma differentiate into tumor endothelial cells.
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