| Project/Area Number |
25462781
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Osaka University (2014-2015)
Hyogo Medical University (2013) |
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Principal Investigator |
Okuyama Hiroomi 大阪大学, 医学(系)研究科(研究院), 教授 (30252670)
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| Co-Investigator(Kenkyū-buntansha) |
Saka Ryuta 大阪大学, 大学院医学系研究科, 助教 (00459190)
Kubo Syuji 兵庫医科大学, 医学部, 教授 (10441320)
Sasaki Takashi 兵庫医科大学, 医学部, 講師 (20388573)
Yanagihara Itaru 大阪府立母子保健総合医療センター, 研究所, 部長 (60314415)
野瀬 聡子 兵庫医科大学, 医学部, 助教 (90467564)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | SP-D / 壊死性腸炎 / サーファクタント蛋白D / 消化管 / サーファクタント / 膵臓 / 肝臓 / toll-like receptor / 胎児 / 腸管粘膜防御機構 |
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| Outline of Final Research Achievements |
Surfactant protein (SP)-A and -D is member of collectin, which plays an important role in innate immunity. The function of SP-A and -D has been studied mainly in the airway. We confirmed the expression of SP-A and -D in the murine fetal intestines and pancreas from the latter of the pregnancy. In human beings, SP-A is localized to airway, so we studied the function of SP-D in the human intestine. Necrotizing enterocolitis (NEC) remains life-threatening disease in the premature infants. NEC may depend on the TLR-4 overexpression in the immature (premature) intestines. Platelet activating factor (PAF) induced TLR-4 overexpression in human intestinal cell line INT407, originally derived from human embryonal intestines. The TLR-4 agonist lipopolysaccharide (LPS) induced IL-8 elevation in TLR-4 overexpressed INT407. Recombinant SP-D (full-length) significantly attenuated the inflammation in the above model. SP-D may have a protective effect in the development of NEC in preterm infants.
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