The examination of brief electrical stimulation for axonal regeneration and its application for diabetes mice

Research Project

Project/Area Number	25462803
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Plastic surgery
Research Institution	Juntendo University
Principal Investigator	Yuhei Natori 順天堂大学, 医学部, 非常勤助手 (80445478)
Co-Investigator(Kenkyū-buntansha)	MIZUNO Hiroshi 順天堂大学, 医学部, 教授 (80343606) AYATO Hayashi 順天堂大学, 医学部, 先任准教授 (10365645)
Research Collaborator	YOSHIZAWA Hidekazu 順天堂大学, 医学部, 助手 (10512593) SENDA Daiki 順天堂大学, 大学院生
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords	Thy1 YFP-16マウス / 短期的電気刺激 / 神経再生 / トランスジェニックマウス / 末梢神経障害 / live imaging / transgenic mouse / peripheral nerve / Thy-1 YFP16マウス / 軸索再生 / 糖尿病 / 神経障害
Outline of Final Research Achievements	We studied nerve regeneration using Thy1 YFP-16 mice. There was correlation between fluorescence intensity and histological axon regeneration in Thy1 YFP-16 mice, with the number of regenerated axons showing the strongest correlation. In brief electrical stimulation, the speed of axon growth in early stage was faster in the 60-minute stimulation group as compared with other groups, suggesting that it may primarily reduce the period where regenerated axon travels beyond the crush site. Moreover, regeneration promoting effect was observed in motor nerves as well as sensory nerves, thus application to various scenes can be expected in the future.