Involvement of fibrocytes for tissue repair in normal and pathological conditions during healing
| Project/Area Number |
25462804
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IMAIZUMI Risa 東邦大学, 医学部, 准修練医 (20453847)
OKADA Emi 東邦大学, 医学部, 准教授 (50318242)
AKASAKA Yoshikiyo 東邦大学, 医学部, 教授 (60202511)
INOMATA Naomi 東邦大学, 医学部, シニアレジデント (10439937)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Fibrocyte / bFGF / 創傷治癒 / 血管新生 / 細胞・組織 / bFGF/FGFR1 system / VEGF / 血管内皮前駆細胞 |
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| Outline of Final Research Achievements |
The precise role of fibrocytes in angiogenesis in wound healing remains unclear. Our aim is to clarify the role of fibrocytes in angiogenesis influenced by basic fibroblast growth factor (bFGF) in wounds. Double immunofluorescence staining focusing on fibrocyte accumulation demonstrated markedly increased formation of capillary-like structures composed of CD34+/pro-collagen I+ fibrocytes in bFGF-treated wounds. However, capillary-like structures formed by CD45+/pro-collagen I+ or CD11b+/pro-collagen I+ fibrocytes were lacking in these wounds. Furthermore, bFGFR1-small interfering RNA (siRNA) injection into wounds followed by bFGF treatment markedly inhibited the formation of capillary-like structures composed of CD34+/pro-collagen I+ fibrocytes, indicating the requirement of bFGF for formation of the capillary-like structures composed of CD34+/pro-collagen I+ fibrocytes in the wounds.
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Report
(4 results)
Research Products
(8 results)
