| Project/Area Number |
25462806
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Kinki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KUSUHARA Hirohisa 近畿大学, 医学部, 講師 (50388550)
MOROTOMI Tadaaki 近畿大学, 医学部, 講師 (10388580)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | PGA / ナノファイバー / 耳介軟骨 / 再生誘導 / 自家移植 / 軟骨再生 / オ－トグラフ / 力学特性 / ポリマー / 細胞播種 |
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| Outline of Final Research Achievements |
In conventional studies on the regeneration of auricle-shaped cartilage in autogenous models using large animals, there were problems with the cartilage regeneration induction capacity and long-term retention of geometric shape. In this study, we sought to improve upon outcome in these regards: a non-woven fabric of polyglycolic acid (PGA) was developed through nanotechnology, evaluating the effect of nanofiber diameter on in vitro cell-seeding efficiency, and in vivo response after implantation in an autogenous large-animal model. Optimal cell maintenance and neocartilage response were seen with PGA fiber diameters of 0.8-3.0 μm for nano-fiber constructs. Biomechanical strength was optimal for PGA fiber diametersin in the same mid-range. These findings demonstrate the potential for nano-scale modulation of auricle-shaped cartilage regeneration in a large-animal model.
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