Lung protective strategy by the gene delivery of lung epitherial grow factor.

• Project/Area Number: 25462826
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Yokohama City University
• Principal Investigator: BABA YASUKO 横浜市立大学, 大学病院, 准教授 (80453041)
• Co-Investigator(Kenkyū-buntansha): KURAHASHI KIYOYASU 横浜市立大学, 市民総合医療センター, 准教授 (50234539) ; YAZAWA TAKUYA 千葉大学, 医学部, 准教授 (50251054)
• Research Collaborator: TOJO KENTARO ; NAGAMINE YUSUKE ; KANEGAE YUMI
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 肺傷害 / 集中治療 / 急性肺傷害

Outline of Final Research Achievements

Several reports shows that　KGF(Keratinocyte Growth Factor) or MSC(Mesenchymal Stem Cell)ameliorates lung injury and promotes the repair processs. In this study, we transfected the KGF expressing adenovirus vector to MSC (KGF-MSC) and we hope that the KGF-MSC could deliver KGF to inflammation site efficiently.
We construsted the adenovirus vector that express the KGF. We confirmed that the KGF-MSC oriented KGF expression in the mouse. As the lung injury model, we established the conditions of the cecal ligation and puncture model and Supra mesenteric aretry(SMA) reperfusion injury model.