| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
The hepatic vein was isolated from the liver in guinea pig and was measured the vasoconstriction and the intracellular Ca2+ concentration. By the transmural nerve stimulation (TNS) transiently increased the tension of the vein. Phenylephrine increased the tension. These responses were not inhibited by nifedipne. Cyclopiazonic acid (CPA) and Y-27632 inhibited the responses. TNS also elicited a transient increase of the cytosolic Ca2+ concentrationx The response was inhibited in presence of tetrodotoxin or phentolamin. Phenylephrine also increased the cytosolic Ca2+. These cytosolic Ca2+ responses were inhibited in presence of CPA. These results suggest that, in the guinea pig hepatic vein, adrenergic nerves stimulate α receptor, which induce the increase of the cytosolic Ca2+ mainly through the intracellular Ca2+ stores or ROK signal, and evoked vasoconstriction to increase the vascular resistance.
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