Manegement of lung epithelial cell response against invasive mechanical ventilation.

• Project/Area Number: 25462828
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Hashimoto Soshi 京都府立医科大学, 医学(系)研究科(研究院), 客員講師 (60515279)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 人工呼吸関連肺障害 / 人工呼吸関連肺損傷 / 集中治療医学 / 人工呼吸 / 肺損傷

Outline of Final Research Achievements

Rats and mice were mechanically ventilated in lower tidal volume (6mL/Kg) or higher tidal volume (20mL/Kg and 40mL/Kg). Lung was removed and wet/dry ratio and albumin leakage was measured. We found increase in wet/dry ratio and albumin leakage in higher tidal volume group. Lung histology showed immune cell invasion after the mechanical ventilation with higher tidal volume.
Using this model, we investigated the expression of inflammatory cytokine and ERM proteins in the lung. We found that mechanical ventilation with higher tidal volume increased the amount of inflammatory cytokines and decreased the expression of ERM protein in the lung.
These result suggested that the relationship between the expression of ERM protein and the inflammatory reaction after the invasive mechanical ventilation.

Research Products

• [Journal Article] mTOR signaling controls VGLUT2 expression to maintain pain hypersensitivity after tissue injury. (2015)
  • Author(s): Izumi Y, Sasaki M, Hashimoto S, Sawa T, Amaya F.
  • Journal Title: Neuroscience. Volume: 308 Pages: 169-179
  • DOI: 10.1016/j.neuroscience.2015.09.013
  • Peer Reviewed / Open Access
• [Journal Article] Perioperative lung protective ventilation in obese patients (2015)
  • Author(s): Fernandez-Bustamante A, Hashimoto S, Serpa Neto A, Moine P, Vidal Melo MF, Repine JE
  • Journal Title: BMC Anesthesiol Volume: 6 Issue: 1 Pages: 56-56
  • DOI: 10.1186/s12871-015-0032-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ten year trend of ventilator settings for acute respiratory distress syndrome: a single institute investigation (2013)
  • Author(s): 橋本壮志, 志馬伸朗, 橋本悟.
  • Journal Title: Journal of the Japanese Society of Intensive Care Medicine Volume: 20 Issue: 2 Pages: 287-288
  • DOI: 10.3918/jsicm.20.287
  • NAID: 130004514527
  • ISSN: 1340-7988, 1882-966X
  • Peer Reviewed
• [Journal Article] Evaluation of semi-quantitative scoring of Gram staining or semi-quantitative culture for the diagnosis of ventilator-associated pneumonia: a retrospective comparison with quantitative culture. (2013)
  • Author(s): Hashimoto S, Shime N.
  • Journal Title: Journal of Intensive Care Volume: 1 Issue: 1 Pages: 2-2
  • DOI: 10.1186/2052-0492-1-2
  • Peer Reviewed
• [Presentation] Lung Inflammation in Patients with Sepsis and ARDS Assessed in vivo by 18F-FDG-PET (2015)
  • Author(s): Hibbert KA, Winkler TW, Hashimoto S, Holland CA, Brait KL, Harris RS, Bajwa EK, Vidal Melo MF
  • Organizer: ANESTHESIOLOGY 2015 Annual Meeting
  • Place of Presentation: San Diego, CA
  • Year and Date: 2015-10-24
  • Int'l Joint Research