| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
In tooth development, the tooth germ is under continuous mechanical stress as it increases in size. The role of PIEZO1 and PIEZO2 are activated by mechanical stimulation and function as nonselective mechanical channels in mice. We investigated the gene expression profiles of Piezo1 and Piezo2 to elucidate their roles in response to mechanical stimulation during tooth development.
Quantitative PCR revealed that Piezo1 and Piezo2 were expressed at all tooth development stages (E15.5, E18.5 and P0 mice). The expression of Piezo2 was remarkably increased at E18.5. In contrast, the expression of Piezo1 at P0 was higher than that at E18.5. The gene expression profiles of Piezo1 and Piezo2 were different in the tooth germ. Although both PIEZO1 and PIEZO2 are known as mechanical stimulation sensors, PIEZO1 and PIEZO2 may possess distinct functions as receptors for mechanical stimulation at each stage of tooth development.
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