Characterization of the type IX secretion system (T9SS)
| Project/Area Number |
25462866
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
SATO Keiko 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (70410579)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 歯周病菌 / 分泌 / プロテアーゼ / 病原因子 / タンパク質分泌 / 歯周病 |
|---|
| Outline of Final Research Achievements |
Porphyromonas gingivalis possesses cysteine proteases named gingipains that act as important virulence factors. Gingipains, which consist of Arg-gingipain A (RgpA), Arg-gingipain B (RgpB) and Lys-gingipain (Kgp), have a characteristic Ig-like domain of the peptidase family C25 that is found at the C-terminal region of the protease domain in almost all family members. When introducing a plasmid encoding either gingipain lacking the common structure or gingipain with an incomplete common structure into a P. gingivalis gingipain-null (rgpA rgpB kgp) mutant, the mutant gingipains were not detected in the cells, whereas mutant gingipains with high molecular mass accumulated in the membrane fraction after introducing the plasmids into an rgpA rgpB kgp htrA mutant, indicating that housekeeping protease degrades the mutant gingipains.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Involvement of an Skp-Like Protein, PGN_0300, in the Type IX Secretion System of Porphyromonas gingivalis.2015
Author(s)
Taguchi Y, Sato K, Yukitake H, Inoue T, Nakayama M, Naito M, Kondo Y, Kano K, Hoshino T, Nakayama K, Takashiba S, Ohara N.
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Journal Title
Infection and Immunity
Volume: 84
Issue: 1
Pages: 230-40
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Identification of an O-antigen chain length regul tor, WzzP, in Porphyromonas gingivalis.2013
Author(s)
Shoji M, Yukitake H, Sato K, Shibata Y, Naito M, Aduse-Opoku J, Abiko Y, Curtis MA, N kayama K.
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Journal Title
Microbiologyopen.Epub
Volume: 10
Issue: 3
Pages: 383-401
DOI
Related Report
Peer Reviewed
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