| Project/Area Number |
25462877
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | ペルオキシダーゼ / キノン / 呼吸鎖 / 侵襲性歯周炎 / 病原因子 / 膜タンパク / 過酸化水素 / 活性酸素 / 阻害剤 |
|---|
| Outline of Final Research Achievements |
Oral pathogen for aggressive periodontitis encodes quinol peroxidase (QPO). Irreversible inactivation of QPO by high concentration of H2O2 exhibited pseudo-first order kinetics. Analyses of initial-rate kinetics and product inhibition of QPO indicate that enzyme catalytic mechanism is explained by a Ping Pong Bi Bi system. In addition, one of the three heme c moieties of QPO is maintained in an oxidized form even at increased ratios of [Q1H2]/[Q1], suggesting that QPO is reduced in the absence of H2O2 and only two of the three heme c moieties are reduced in the presence of high concentration of the Q1H2. We also found that ascochlorin (competitive-type, Ki=24.7 nM), ilicicolin B (mixed-type, Ki=576 nM), ilicicolin F (competitive-type, Ki=19.5 nM), N-heptyl-quinoline-N-oxide (competitive-type, Ki=1.0 microM), 2-heptyl-3-hydroxy-4-quinolone (noncompetitive-type, Ki=7.0 microM) are potent inhibitor for QPO.
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