Pathological analysis of autoimmune sialadenitis using human dental pulp
| Project/Area Number |
25462878
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Ryo Koufuchi 鶴見大学, 歯学部, 講師 (10298268)
Mishima Kenji 昭和大学, 歯学部, 教授 (50275343)
Inoue Hiroko 日本薬科大学, 薬学部, 准教授 (50367306)
Ichiro Sito 鶴見大学, 歯学部, 教授 (60147634)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 唾液腺 / シェーグレン症候群 / 歯髄細胞 / 分化転換 |
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| Outline of Final Research Achievements |
To investigate mechanism of autoimmune sialoadenitis, transdifferentiation from pulp cells to salivary gland cell was tested using normal and Sjogren syndrome human dental pulp tissues. Co-culture of normal pulp cells and salivary gland cell was carried out and expression of salivary gland cell was investigated using RT-PCR. Dental pulp cell expressed aquaporin-5 (AQP-5) after co-culture of salivary gland cells. However, other markers such as amylase were not detected. The result suggests that transdifferentiation from dental pulp to salivary gland cells is difficult using co-culture technique.
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Report
(4 results)
Research Products
(15 results)
