Study on colonization mechanism of periodontal pathogens: preparation of infection experiment under anaerobic conditions and analysis of fimbrial functions for colonization

• Project/Area Number: 25462880
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Aichi Gakuin University
• Principal Investigator: Nagano Keiji 愛知学院大学, 歯学部, 講師 (60367620)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 歯周病 / 歯肉上皮細胞 / 嫌気培養 / 付着 / トレポネーマ / 運動 / べん毛 / 歯周病原性細菌 / 侵入 / 蛍光顕微鏡 / 電子顕微鏡 / 歯周病原因細菌 / Porphyromonas gingivalis / 線毛 / FimA

Outline of Final Research Achievements

Periodontal disease is caused by multi-species of bacteria colonizing the periodontal tissues. Some species directly adhere to the gingival epithelial cells, but the mechanism is not well understood. Periodontitis-associated bacteria generally grow in an oxygen-free (anaerobic) environment. However, studies on adhesion to gingival epithelial cells have been performed under aerobic conditions. Therefore, in this study, it was conducted to establish a method of the infection experiment under anaerobic conditions. In addition, several strains of periodontitis-associated and anaerobic bacterium Treponema denticola were comparatively analyzed for some typical pathogenicities including adhesive activity to the gingival epithelia.

Research Products

• [Journal Article] A major fimbrilin variant of Mfa1 fimbriae in Porphyromonas gingivalis (2015)
  • Author(s): K. Nagano, Y. Hasegawa, Y. Yoshida, and F. Yoshimura
  • Journal Title: J. Dent. Res Volume: 94 Issue: 8 Pages: 1143-1148
  • DOI: 10.1177/0022034515588275
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Draft Genome Sequence of Porphyromonas gingivalis strain Ando expressing a 53-kilodalton-type fimbrilin variant of Mfa1 fimbriae (2015)
  • Author(s): Goto T, Nagano K, Hirakawa H, Tanaka K, Yoshimura F
  • Journal Title: Genome Announc Volume: 3 Issue: 6
  • DOI: 10.1128/genomea.01292-15
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Characterization of Treponema denticola mutants defective in the major antigenic proteins, Msp and TmpC. (2014)
  • Author(s): Abiko Y, Nagano K, Yoshida Y, Yoshimura F.
  • Journal Title: PLoS One Volume: 17;9(11) Issue: 11 Pages: e113565-e113565
  • DOI: 10.1371/journal.pone.0113565
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola (2014)
  • Author(s): Abiko Y, Nagano K, Yoshida Y, Yoshimura F
  • Journal Title: PLoS One Volume: 21;9(2) Issue: 2 Pages: e89051-e89051
  • DOI: 10.1371/journal.pone.0089051
  • Peer Reviewed / Open Access
• [Presentation] Comparative analysis of the motility, protease and adherence activity of Treponema denticola strains (2016)
  • Author(s): Nagano K, Hasegawa Y, Yoshida Y, Yoshimura F.
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター（大阪）
  • Year and Date: 2016-03-23
• [Presentation] 口腔スピロヘータTreponema denticolaの株間における性状比較解析 (2016)
  • Author(s): 吉村文信、永野恵司、長谷川義明、吉田康夫
  • Organizer: 平成27年度 東海乳酸菌研究会総会
  • Place of Presentation: 中日ビル（名古屋）
  • Year and Date: 2016-02-06
• [Presentation] これまでの研究実績 (2015)
  • Author(s): 永野 恵司
  • Organizer: 第52 回日本細菌学会中部支部総会
  • Place of Presentation: 名古屋市立大学（名古屋）
  • Year and Date: 2015-10-24