Study for elucidation of the functional significance and molecular basis of area postrema neurons relating to the induction of emesis and the regulation of food intake.
| Project/Area Number |
25462883
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
FUNAHASHI Makoto 北海道大学, 歯学研究科(研究院), 教授 (80221555)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAI Yoshiyuki 北海道大学, 歯学研究科, 助教 (40344519)
MAEZAWA Hitoshi 北海道大学, 歯学研究科, 助教 (80567727)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 最後野 / 嘔吐 / 悪心 / 摂食 / 電気生理学 / 行動科学 / コレシストキニン / ヒスチジン / area postrema / food intake / CCK / rats / H channel / patch-clamp / 摂食抑制 / 脳スライス / パッチクランプ / Hチャネル |
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| Outline of Final Research Achievements |
As neurons responding to amylin and/or CCK-8 are considered to be a neuron group that participates in the regulation of food intake, we examined responsiveness of area postrema neurons to these peptide hormones. Excitatory responses to amylin were found in Ih(-) neurons alone. Excitatory responses to CCK-8 were found in Ih(-) neurons (n=38/42) and Ih(+) neurons (n=4/42). These data indicate that Ih(-) neurons in the area postrema contribute in the regulation of food intake. We also demonstrated that amylin and CCK-8 receptors were located on the presynaptic membrane, and regulate the transmitter, glutamate, release. The present study suggests two possible regulation mechanism of food intake, such as anorexia with visceral uncomfortable feeling (nausea) or appetite suppression without nausea. Ih(+) neurons and Ih(-) neurons in the area postrema may contribute to organizing neuron network for different modulation system of food intake.
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Report
(4 results)
Research Products
(22 results)
