The study on the mechanism of nerve paralysis and its application for the therapy

• Project/Area Number: 25462885
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Osaka University
• Principal Investigator: Toyoda Hiroki 大阪大学, 歯学研究科, 准教授 (00432451)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 漏洩カリウムチャネル / 運動ニューロン / 一酸化窒素 / cGMP / 三叉神経 / 顔面神経 / 入力抵抗 / スパイク / 神経麻痺 / TASKチャネル / 膜興奮性 / イオンチャネル

Outline of Final Research Achievements

In the present study, we studied the mechanism of nerve paralysis by focusing on the role of leak K+ channels expressed in motoneurons. Following application of 8-Br-cGMP, input resistances were decreased and spike onsets were delayed in small-sized motoneurons (< 20 μm), while input resistances and spike onsets remained almost unchanged in large-sized motoneurons (> 35 μm). These results suggest that activation of NO-cGMP-PKG pathway alters membrane excitability of motoneurons through the modulation of activities of leak K+ currents. Such neural mechanisms are likely to be involved in the generation of neural paralysis.

Research Products

• [Journal Article] A distinct functional distribution of α and γ motoneurons in the rat trigeminal motor nucleus. (2017)
  • Author(s): Morita-Isogai, Y., Sato, H., Saito, M., Kuramoto, E., Yin, D., Kaneko, T., Yamashiro, T., Takada, K., Oh, S.B., Toyoda, H., & Kang, Y.
  • Journal Title: Brain Structure and Function Volume: - Issue: 7 Pages: 1-9
  • DOI: 10.1007/s00429-017-1400-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Possible Role of TASK Channels in Rank-Ordered Recruitment of Motoneurons in the Dorsolateral Part of the Trigeminal Motor Nucleus. (2016)
  • Author(s): Okamoto K, Emura N, Sato H, Fukatsu Y, Saito M, Tanaka C, Morita Y, Nishimura K, Kuramoto E, Xu Yin D, Furutani K, Okazawa M, Kurachi Y, Kaneko T, Maeda Y, Yamashiro T, Takada K, Toyoda H, Kang Y.
  • Journal Title: eNeuro Volume: 3(3) Issue: 3 Pages: 3-3
  • DOI: 10.1523/eneuro.0138-16.2016
  • Peer Reviewed / Open Access
• [Journal Article] A role of CB1R in inducing θ-rhythm coordination between the gustatory and gastrointestinal insula. (2016)
  • Author(s): Kang, Y., Sato, H., Saito, M.,Yin, D., Park, S.K., Oh, S.B., Bae, Y.C. & Toyoda, H.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 32529-32529
  • DOI: 10.1038/srep32529
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Brain Mechanisms Underlying the Perception of Pungent Taste of Capsaicin and the Subsequent Autonomic Responses (2016)
  • Author(s): Shinpei Kawakami, Hajime Sato, Akihiro T. Sasaki, Hiroki C. Tanabe, Yumiko Yoshida, Mitsuru Saito, Hiroki Toyoda, Norihiro Sadato and Youngnam Kang
  • Journal Title: Frontiers in Human Neuroscience Volume: 9 Pages: 1-16
  • DOI: 10.3389/fnhum.2015.00720
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Role of leak potassium channels in pain signaling (2015)
  • Author(s): Li, X.Y. et al.
  • Journal Title: Brain Research Bulletin Volume: 119 Pages: 73-79
  • DOI: 10.1016/j.brainresbull.2015.08.007
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Teeth clenching reduces arm abduction force (2014)
  • Author(s): Sato H, Kawano T, Saito M, Toyoda H, Maeda Y, Tiirker KS, Kang Y
  • Journal Title: Experimental Brain Research Volume: (Epub ahead of print) Issue: 7 Pages: 2281-2291
  • DOI: 10.1007/s00221-014-3919-8
  • Peer Reviewed / Open Access
• [Journal Article] Enhanced desensitization followed by unusual resensitization in GABAA receptors in phospholipase C-related catalytically inactive protein-1/2 double-knockout mice (2014)
  • Author(s): Toyoda H, Saito M, Sato H, Tanaka T, Ogawa T, Yatani H, Kawano T, Kanematsu T, Hirata M, Kang Y
  • Journal Title: Pflugers Archiv - European Journal of Physiology Volume: (Epub ahead of print) Issue: 2 Pages: 267-284
  • DOI: 10.1007/s00424-014-1511-5
  • Peer Reviewed / Open Access
• [Journal Article] GABAB receptor-mediated presynaptic inhibition reverses inter-columnar covariability of synaptic actions by intracortical axons in the rat barrel cortex (2013)
  • Author(s): Sato H, Toyoda H, Saito M, Kobayashi M, Althof D, Kulik A, Kang Y
  • Journal Title: The European Journal of Neuroscience Volume: 37 Issue: 2 Pages: 190-202
  • DOI: 10.1111/ejn.12041
  • Peer Reviewed
• [Journal Article] A mathematical model of negative covariability of inter-columnar excitatory synaptic actions caused by presynaptic inhibition (2013)
  • Author(s): Saito M, Tanaka T, Sato H, Toyoda H, Aoyagi T, Kang Y
  • Journal Title: The European Journal of euroscience Volume: 38 Issue: 7 Pages: 2999-3007
  • DOI: 10.1111/ejn.12299
  • Peer Reviewed
• [Presentation] アナンダミドにより島皮質味覚野と胃腸自律領野間にネットワークオシレーションが誘発される (2017)
  • Author(s): 豊田博紀、佐藤元、尹東旭、姜英男
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 浜松アクトシティコングレスセンター（静岡県浜松市）
• [Presentation] 島皮質オシレーションと味覚-摂食機能連関 (2016)
  • Author(s): 豊田博紀
  • Organizer: 第58回歯科基礎医学会学術大会・総会サテライトシンポジウム
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
• [Presentation] The network oscillation in the insular cortex (2015)
  • Author(s): Toyoda, H. et al.
  • Organizer: The 10th International conference of neurons and brain diseases
  • Place of Presentation: Xi'an Jiaotong University (Xi'an, China)
  • Year and Date: 2015-06-23
  • Int'l Joint Research
• [Presentation] The role of TASK channels in rank-ordered recruitment of trigeminal jaw-closing motoneurons (2015)
  • Author(s): Toyoda, H. et al.
  • Organizer: The International Symposium on Neuroscience in Orofacial sensory-motor functions 2015
  • Place of Presentation: 大阪大学中之島センター（大阪）
  • Year and Date: 2015-05-11
  • Int'l Joint Research
• [Presentation] ラットバレル皮質におけるカイニン酸誘発性オシレーション (2015)
  • Author(s): 豊田博紀 他
  • Organizer: 第92回日本生理学会大会・第120回日本解剖学会総会全国学術集会合同大会
  • Place of Presentation: 神戸国際会議場(神戸)
  • Year and Date: 2015-03-21
• [Presentation] Enhancement of lateral inhibition in the mouse barrel cortex by deletion of phospholipase C-related catalytically inactive protein-1/2 (2014)
  • Author(s): Toyoda H., et al.
  • Organizer: The 44th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Walter E. Washington Convention Center（Washington DC）
  • Year and Date: 2014-11-17
• [Presentation] 大脳皮質体性感覚野バレル皮質領野におけるカラム間の周期的同期化機構 (2014)
  • Author(s): 豊田博紀 他
  • Organizer: 第56回歯科基礎医学会学術大会・総会サテライトシンポジウム
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2014-09-25
• [Presentation] 大脳皮質体性感覚野バレル皮質領野におけるカラム間の周期的同期化機構 (2014)
  • Author(s): 豊田博紀 他
  • Organizer: 第56回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2014-09-25
• [Presentation] TASKチャネルによる咬筋運動ニューロンの序列動員生成機序 (2014)
  • Author(s): 豊田博紀 他
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: 横浜国際会議場（横浜）
  • Year and Date: 2014-09-11
• [Presentation] バレル皮質錐体細胞においてPRIP遺伝子欠損により細胞外シナプスGABAA受容体の発現が増加する (2013)
  • Author(s): 豊田博紀 他
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都国際会館（京都）
• [Presentation] Phospholipase C-related inactive protein regulates the phasic and tonic inhibition in the barrel cortex (2013)
  • Author(s): Toyoda, H. et al
  • Organizer: The 11th Japan-Korea Joint Symposium of Brain Sciences, and Cardiac and Smooth Muscles
  • Place of Presentation: 浜松アクトシティ（浜松）
• [Presentation] PRIP1/2蛋白の発現抑制はバレル皮質錐体細胞において一過性GABA抑制を強化する (2013)
  • Author(s): 豊田博紀 他
  • Organizer: 第55回歯科基礎医学会
  • Place of Presentation: 岡山コンベンションセンター（岡山）
• [Presentation] Phospholipase C-related inactive proteins are involved in the regulation of phasic and tonic inhibition in the barrel cortex (2013)
  • Author(s): Toyoda, H. et al
  • Organizer: 43rd Society for Neuroscience
  • Place of Presentation: San Diego convention center（San Diego）