Expression and functional analysis of MSX homeobox gene family in dental pulp

• Project/Area Number: 25462889
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: FUJIMOTO KATSUMI 広島大学, 医歯薬保健学研究院(歯), 助教 (40294566)
• Research Collaborator: GOTO Noriko 広島大学, 病院(歯), 歯科診療医 (10735137)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 歯髄細胞 / MSX1 / 骨芽細胞分化 / 象牙芽細胞分化 / 歯髄 / MSX1/2

Outline of Final Research Achievements

We examined that the role of MSX1 in regulating osteogenic/odontogenic differentiation of dental pulp stem cells (DPSCs). Knockdown of MSX1 suppressed the expression of osteoblast-related genes, alkaline phosphatase activity and calcification in DPSCs under osteogenic conditions, suggesting that MSX1 positively regulates osteogenic/odontogenic differentiation. MSX1 proteins were intensely expressed in odontoblasts of the roots and pulpal mesenchymal cells surrounding the blood vessels in the maxillary molar of 3 week-old mice. In addition, we performed gene expression microarray and ChIP-seq analysis to identify candidate MSX1 target genes.

Research Products

• [Journal Article] DEC2 is a negative regulator for the proliferation and differentiation of chondrocyte lineage-committed mesenchymal stem cells (2016)
  • Author(s): Sasamoto, T., Fujimoto, K., Kanawa, M., Kimura, J., Takeuchi, J., Harada, N., Goto, N., Kawamoto, T., Noshiro, M., Suardita, K., Tanne, K., Kato, Y.
  • Journal Title: Int. J. Mol. Med Volume: 印刷中 Issue: 3 Pages: 876-884
  • DOI: 10.3892/ijmm.2016.2660
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells (2015)
  • Author(s): Fujii, S. Fujimoto, K. Goto, N. Kanawa, M. Kawamoto, T. Pan, H. Srivatanakul, P. Rakdang, W. Pornprasitwech, J. Saskianti, T. Suardita, K. Nishimura, F. Kato, Y.
  • Journal Title: Biomed Rep. Volume: 3 Issue: 4 Pages: 566-572
  • DOI: 10.3892/br.2015.456
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] DEC2-E4BP4 heterodimer represses the trancriptional enhancer activity of the EE element in the Per2 promoter (2015)
  • Author(s): Tanoue S, Fujimoto K, Myung J, Hatanaka F, Kato Y, Takumi T
  • Journal Title: Front Neurol Volume: 6 Pages: 166-166
  • DOI: 10.3389/fneur.2015.00166
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] <b>Basic helix-loop-helix transcription factor DEC1 regulates the cisplatin-induced apoptotic pathway of human esophageal cancer </b><b>cells </b> (2015)
  • Author(s): Seino H
  • Journal Title: Biomedical Research Volume: 36 Issue: 2 Pages: 89-96
  • DOI: 10.2220/biomedres.36.89
  • NAID: 130005065284
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Involvement of c-Myc in the proliferation of MCF-7 human breast cancer cells induced by bHLH transcription factor DEC2. (2015)
  • Author(s): Wu Y
  • Journal Title: Int J Mol Med. Volume: 35 Issue: 3 Pages: 815-20
  • DOI: 10.3892/ijmm.2014.2042
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] DEC1/STRA13/SHARP2 and DEC2/SHARP1 coordinate physiological processes, including circadian rhythms in response to environmental stimuli (2014)
  • Author(s): Kato Y, Kawamoto T, Fujimoto K, Noshiro M
  • Journal Title: Curr. Top. Dev. Biol. Volume: 110 Pages: 339-372
  • DOI: 10.1016/b978-0-12-405943-6.00010-5
  • ISBN: 9780124059436
  • Peer Reviewed
• [Journal Article] A novel protein, CHRONO, functions as a core component of the mammalian circadian clock. (2014)
  • Author(s): Goriki, A., Hatanaka, F., Myung J., Kim, J., Yoritaka, T., Tanoue, S., Abe, T., Kiyonari, H., Fujimoto, K., Kato, Y., Todo, T., Matsubara, A., Forger, D. and Takumi, T.
  • Journal Title: PLoS Biol. Volume: 12 Issue: 4 Pages: e1001839-e1001839
  • DOI: 10.1371/journal.pbio.1001839
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Different Circadian Expression of Major Matrix-Related Genes in Various Types of Cartilage : Modulation by Light-Dark Conditions (2013)
  • Author(s): Honda KK, Kawamoto T, Ueda HR, Nakashima A, Ueshima T, Yamada RG, Nishimura M, Oda R, Nakamura S, Kojima T, Noshiro M, Fujimoto K, Hashimoto S, Kato Y
  • Journal Title: J Biochem Volume: 154(4) Issue: 4 Pages: 373-81
  • DOI: 10.1093/jb/mvt068
  • NAID: 40019817581
  • Peer Reviewed
• [Presentation] MSX1 down-regulates the expression of cholesterol synthesis-related genes in dental pulp stem cells (2015)
  • Author(s): 五藤紀子、藤本勝巳、藤井紗貴子、依田浩子、大島勇人、河本 健、能城光秀、宿南知佐、香西克之、加藤幸夫
  • Organizer: 第38回日本分子生物学会年会第88回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県・神戸市）
  • Year and Date: 2015-12-01
• [Presentation] ホメオボックス型転写因子MSX1による歯髄幹細胞分化制御 (2015)
  • Author(s): 藤本勝巳、五藤紀子、河本 健、能城光秀、宿南知佐、香西克之、加藤幸夫
  • Organizer: 第99回広島大学歯学会例会 併催 第54回広島県歯科医学会 併催 日本歯科技工学会中国・四国支部第10回学術大会
  • Place of Presentation: 広島県歯科医師会館（広島県・広島市）
  • Year and Date: 2015-11-08
• [Presentation] The improved performance of mesenchymal stem cell cultures by fibronectin and mixed self-assembled monolayers under serum-free conditions (2014)
  • Author(s): Veronica Sainik Ronald, Masami Kanawa, Isao Hirata, Koichi Kato, Katsumi Fujimoto, Chisa Shukunami, Yukio Kato
  • Organizer: 第98回広島大学歯学会例会
  • Place of Presentation: 広島
  • Year and Date: 2014-11-09
• [Presentation] ホメオボックス型転写因子MSX1による歯髄幹細胞の象牙芽細胞／骨芽細胞分化制御 (2014)
  • Author(s): 五藤紀子、藤本勝巳、依田浩子、大島勇人、河本健、能城光秀、宿南知佐、香西克之、加藤幸夫
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] ホメオボックス型転写因子MSX1による歯髄幹細胞の象牙芽細胞／骨芽細胞分化の制御 (2014)
  • Author(s): 五藤紀子，藤本勝巳，依田浩子，大島勇人，河本健，能城光秀，宿南知佐，香西克之，加藤幸夫
  • Organizer: 第15回運動器科学研究会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-05
• [Presentation] １： Genome-wide analysis of MSX1 target genes in stem cells from human exfoliated deciduous teeth (2013)
  • Author(s): Noriko Goto, Katsumi Fujimoto, Sakiko Fuii, Takeshi Kawamoto, Mitsuhide Noshiro, Katsuyuki Kouzai, Yukio Kato
  • Organizer: the 24th Congress of International Association of Paediatric Dentistry (IAPD)
  • Place of Presentation: 韓国、ソウル
• [Presentation] ３： The identification of MSX1 target genes in stem cells from human exfoliated deciduous teeth (SHED) (2013)
  • Author(s): 五藤紀子、藤本勝巳、今村芹佳、藤井紗貴子、河本健、能城光秀、香西克之、加藤幸夫
  • Organizer: 第46回広島大学歯学会総会
  • Place of Presentation: 広島
• [Presentation] ２： The functional role of MSX1 in stem cells from human exfoliated deciduous teeth (SHED) (2013)
  • Author(s): Noriko Goto, Katsumi Fujimoto, Veronica Sainik Ronald, Sakiko Fujii, Serika Imamura, Takeshi Kawamoto. Mitsuhide Noshiro, Katsuyuki Kozai, Yukio Kato
  • Organizer: 5th Hiroshima Conference on Education and Science
  • Place of Presentation: 広島