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Analysis of dipeptidyl peptidase gene group

Research Project

Project/Area Number 25462893
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional basic dentistry
Research InstitutionNagasaki University

Principal Investigator

KOBAYAKAWA Takeshi  長崎大学, 医歯薬学総合研究科(歯学系), 技術専門職員 (10153587)

Co-Investigator(Kenkyū-buntansha) NEMOTO Takayuki K  長崎大学, 医歯薬学総合研究科(歯学系), 教授 (90164665)
NEMOTO Yuko (OHARA Yuko)  長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (10164667)
Co-Investigator(Renkei-kenkyūsha) KIMURA Shigenobu  岩手医科大学, 歯学部, 教授 (10177917)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords歯周病原性細菌 / Porphyromonas gingivalis / レサズリン / 蛍光 / アミノ酸代謝 / トランスポーター / 歯周病原菌 / P. gingivalis / ジペプチジルペプチダーゼ / 代謝活性 / 歯周病菌 / P.gingivalis / プロテアーゼ
Outline of Final Research Achievements

We investigated the mechanism of amino acid-uptake in a periodontopathic bacterium, Porphyromonas gingivalis. Bacterial growth was markedly reduced in the 4 dpp genes disrupted strain. This result indicated that the DPP activity plays a crucial role in the bacterial growth and energy metabolism. We revealed that the bacterial metabolic activity and amino acid uptake could be measured by the analysis with Resazurin. By using this procedure, we found that P. gingivalis incorporated dipeptides as the nutrient, and that this route is indispensable for the growth of this bacterium.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2015 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results)

  • [Journal Article] Identification and characterization of prokaryotic dipeptidyl-peptidase 5 from Porphyromonas gingivalis.2014

    • Author(s)
      Ohara-Nemoto Y, Rouf SMA, Naito M, Yanase A, Tetsuo F, Ono T, Kobayakawa T, Shimoyama Y, Kimura S, Nakayama K, Saiki K, Konishi K, Nemoto TK.
    • Journal Title

      J. Biol. Chem.

      Volume: 289 Issue: 9 Pages: 5438-5448

    • DOI

      10.1074/jbc.m113.527333

    • NAID

      120006986777

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 非RI蛍光試薬によるPorphyromonas gingivalisのアミノ酸とジペプチドの取り込みの評価2015

    • Author(s)
      小早川健,根本優子,馬場友巳,根本孝幸
    • Organizer
      第57回歯科基礎医学会学術大会・総会
    • Place of Presentation
      新潟市 朱鷺メッセ
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 歯周病原性細菌の新規オリゴペプチダーゼの基質特異性と分子集合解離機構2014

    • Author(s)
      根本孝幸,小野俊雄,下山佑,木村重信,根本 優子
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Year and Date
      2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 歯周病原性細菌の新規オリゴペプチダーゼ:基質特異性と分子集合性2014

    • Author(s)
      根本孝幸,根本優子,小野俊雄, 下山佑,木村重信
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Identification and Characterization of Dipeptidyl-Peptidase V as a Member for Dipeptide Production in Porphyromonas gingivalis.2013

    • Author(s)
      根本孝幸,ラオフ シャーク ムハマド アブドゥー,根本優子,下山佑,木村重信
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Porphyromonas endodontalisの発現するユニークなジペプチジルペプチダーゼ(DPP)VおよびDPP7.2013

    • Author(s)
      柳瀬絵美,根本優子,下山佑,木村重信,馬場友巳,根本孝幸
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山コンベンションセンター(岡山県岡山市)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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