Analysis of dipeptidyl peptidase gene group

• Project/Area Number: 25462893
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Nagasaki University
• Principal Investigator: KOBAYAKAWA Takeshi 長崎大学, 医歯薬学総合研究科(歯学系), 技術専門職員 (10153587)
• Co-Investigator(Kenkyū-buntansha): NEMOTO Takayuki K 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (90164665) ; NEMOTO Yuko (OHARA Yuko) 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (10164667)
• Co-Investigator(Renkei-kenkyūsha): KIMURA Shigenobu 岩手医科大学, 歯学部, 教授 (10177917)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 歯周病原性細菌 / Porphyromonas gingivalis / レサズリン / 蛍光 / アミノ酸代謝 / トランスポーター / 歯周病原菌 / P. gingivalis / ジペプチジルペプチダーゼ / 代謝活性 / 歯周病菌 / P.gingivalis / プロテアーゼ

Outline of Final Research Achievements

We investigated the mechanism of amino acid-uptake in a periodontopathic bacterium, Porphyromonas gingivalis. Bacterial growth was markedly reduced in the 4 dpp genes disrupted strain. This result indicated that the DPP activity plays a crucial role in the bacterial growth and energy metabolism. We revealed that the bacterial metabolic activity and amino acid uptake could be measured by the analysis with Resazurin. By using this procedure, we found that P. gingivalis incorporated dipeptides as the nutrient, and that this route is indispensable for the growth of this bacterium.

Research Products

• [Journal Article] Identification and characterization of prokaryotic dipeptidyl-peptidase 5 from Porphyromonas gingivalis. (2014)
  • Author(s): Ohara-Nemoto Y, Rouf SMA, Naito M, Yanase A, Tetsuo F, Ono T, Kobayakawa T, Shimoyama Y, Kimura S, Nakayama K, Saiki K, Konishi K, Nemoto TK.
  • Journal Title: J. Biol. Chem. Volume: 289 Issue: 9 Pages: 5438-5448
  • DOI: 10.1074/jbc.m113.527333
  • NAID: 120006986777
  • Peer Reviewed / Open Access
• [Presentation] 非RI蛍光試薬によるPorphyromonas gingivalisのアミノ酸とジペプチドの取り込みの評価 (2015)
  • Author(s): 小早川健，根本優子，馬場友巳，根本孝幸
  • Organizer: 第57回歯科基礎医学会学術大会・総会
  • Place of Presentation: 新潟市 朱鷺メッセ
  • Year and Date: 2015-09-11
• [Presentation] 歯周病原性細菌の新規オリゴペプチダーゼの基質特異性と分子集合解離機構 (2014)
  • Author(s): 根本孝幸，小野俊雄，下山佑，木村重信，根本 優子
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2014-10-18
• [Presentation] 歯周病原性細菌の新規オリゴペプチダーゼ：基質特異性と分子集合性 (2014)
  • Author(s): 根本孝幸，根本優子，小野俊雄, 下山佑，木村重信
  • Organizer: 第56回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2014-09-27
• [Presentation] Identification and Characterization of Dipeptidyl-Peptidase V as a Member for Dipeptide Production in Porphyromonas gingivalis. (2013)
  • Author(s): 根本孝幸，ラオフ シャーク ムハマド アブドゥー，根本優子，下山佑，木村重信
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市）
• [Presentation] Porphyromonas endodontalisの発現するユニークなジペプチジルペプチダーゼ(DPP)VおよびDPP7. (2013)
  • Author(s): 柳瀬絵美，根本優子，下山佑，木村重信，馬場友巳，根本孝幸
  • Organizer: 第55回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山コンベンションセンター(岡山県岡山市)