Study of a novel enzymatic group of dipeptidyl peptidases and their cellular localization
Project/Area Number |
25462894
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Functional basic dentistry
|
Research Institution | Nagasaki University |
Principal Investigator |
OHARA-NEMOTO Yuko 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (10164667)
|
Co-Investigator(Kenkyū-buntansha) |
NEMOTO Takayuki K. 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (90164665)
KIMURA Shigenobu 岩手医科大学, 歯学部, 教授 (10177917)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | Porphyromonas gingivalis / ジペプチジルペプチダーゼ / アミノ酸代謝 / ペリプラズム / 歯周病 / ペプチダーゼ / 歯周病原性細菌 / 嫌気性菌 / 糖非発酵性細菌 / 口腔細菌 / 酵素 |
Outline of Final Research Achievements |
We examined enzymatic activity and cellular localization of a group of dipeptidyl peptidases (DPP) in a periodontopathic bacterium, Porphyromonas gingivalis. This study revealed that DPP5, which had been discovered in fungi, was expressed in the bacterium, and that a novel peptidase, acylpeptidyl oligopeptidase (AOP), was identified. Ortholog search demonstrated wide spreading of the two exopeptidases from bacteria, archea to eukaryote. DPP5 cleaves X-Ala dipeptides from the N-terminus of oligopeptides and AOP liberates di- and tri-peptides from N-terminally blocked ones. Both peptidases were expressed in the periplasmic space of the bacterium. Consequently, we proposed that dipeptide-producing exopeptidases including DPP4, DPP5, DPP7, DPP11 and AOP are crucial for the growth and pathogenicity of P. gingivalis.
|
Report
(4 results)
Research Products
(27 results)
-
-
[Journal Article] Identification and characterization of prokaryotic dipeptidyl-peptidase 5 from Porphyromonas gingivalis.2014
Author(s)
Ohara-Nemoto Y, Rouf SMA, Naito M, Yanase A, Tetsuo F, Ono T, Kobayakawa T, Shimoyama Y, Kimura S, Nakayama K, Saiki K, Konishi K, Nemoto TK.
-
Journal Title
J. Biol. Chem.
Volume: 289
Issue: 9
Pages: 5438-5448
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] In vitro processing of glutamyl endopeptidase proenzymes from Enterococcus faecalis and importance of N-terminal residue in enzyme catalysis.2013
Author(s)
Rouf, S. M., Ohara-Nemoto, Y., Ono, T., Shimoyama, Y., Kimura, S. and Nemoto, T. K.
-
Journal Title
Adv. Biochem.
Volume: 1
Issue: 5
Pages: 73-80
DOI
Related Report
Peer Reviewed
-
[Journal Article] Phenylalanine 664 of dipeptidyl peptidase (DPP) 7 and Phenylalanine 671 of DPP11 mediate preference for P2-position hydrophobic residues of a substrate.2013
Author(s)
Rouf, S. M., Ohara-Nemoto, Y., Ono, T., Shimoyama, Y., Kimura, S. and Nemoto, T. K.
-
Journal Title
FEBS Open Bio.
Volume: 3
Issue: 1
Pages: 177-183
DOI
NAID
Related Report
Peer Reviewed
-
[Journal Article] Measurement of protease activity of exfoliative toxin A using synthetic peptidyl substrates and correlation between in vivo and in vitro activities.2013
Author(s)
Tachi, M. T., Ohara-Nemoto, Y., Baba, T.T., Kobayakawa, T., Fujita, S., Ikeda, T., Ayuse, T., Oi, K., Nemoto, T.K.
-
Journal Title
Acta Med. Nagasaki.
Volume: 58
Pages: 41-48
NAID
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Dipeptide production in Porphyromonas gingivalis mediated by dipeptidyl peptidases and gingipains.2013
Author(s)
Ohara-Nemoto, Y., Abdur-Rouf, S. M., Shimoyama, Y., Kimura, S., and Nemoto, T. K.
Organizer
113rd General meeting of ASM
Place of Presentation
The Colorado Convention Center (Denver, USA)
Related Report
-
[Presentation] Distribution of Porphyromonas gingivalis fimA genotypes in the children’s plaques.2013
Author(s)
Shioyama, Y., Ohara-Nemoto, Y., Kimura, M., Sasaki, M., Tanaka, M., and Kimura, S.
Organizer
113rd General meeting of ASM
Place of Presentation
The Colorado Convention Center (Denver, USA)
Related Report
-
-
-
-
-