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Functional analysis of the pioneer transcription factor FoxO1 in keratinocytes

Research Project

Project/Area Number 25462906
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional basic dentistry
Research InstitutionFukuoka Dental College

Principal Investigator

HATTA MITSUTOKI  福岡歯科大学, 口腔歯学部, 講師 (30344518)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsFoxO1 / 遺伝子発現
Outline of Final Research Achievements

In this study, we generated a tetracycline-inducible FoxO1 expressing keratinocyte cell line (Tet-On Ty1-FoxO1 cells), in order to clarify the functional roles of FoxO1 in keratinocytes. Tet-On Ty1-FoxO1 cells were treated with doxycycline to induce the expression of FoxO1, and then subjected to a global gene expression analysis. We identified FoxO1-regulated genes in keratinocytes, and confirmed the FoxO1 binding to genomic DNA of the target genes. Furthermore, we found that 3-deazaneplanocin A reduced the protein levels of FoxO1 in an oral squamous cell carcinoma cell line.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (3 results)

All 2016 2015

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (1 results)

  • [Journal Article] Mechanistic insight into the aberrant silencing of the keratin 13 gene in oral squamous cell carcinoma cells.2016

    • Author(s)
      Hatta M., Arita S., Yamazaki J.
    • Journal Title

      J. Oral Biosci.

      Volume: 58 Issue: 2 Pages: 45-49

    • DOI

      10.1016/j.job.2016.01.001

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 3-Deazaneplanocin A suppresses aggressive phenotype-related gene expression in an oral squamous cell carcinoma cell line.2015

    • Author(s)
      Hatta M., Naganuma K., Kato K., Yamazaki J.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 468(1-2) Issue: 1-2 Pages: 269-273

    • DOI

      10.1016/j.bbrc.2015.10.115

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] 3-Deazaneplanocin Aは口腔がん細胞の遺伝子発現パターンを変化させる2015

    • Author(s)
      八田 光世、永沼 香織、有田 晴一、石井 太郎、大久保 つや子、山﨑 純
    • Organizer
      第57回歯科基礎医学会学術大会
    • Place of Presentation
      朱鷺メッセ新潟コンベンションセンター(新潟県新潟市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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