Functional analysis of the pioneer transcription factor FoxO1 in keratinocytes

Research Project

Project/Area Number	25462906
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Functional basic dentistry
Research Institution	Fukuoka Dental College
Principal Investigator	HATTA MITSUTOKI 福岡歯科大学, 口腔歯学部, 講師 (30344518)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	FoxO1 / 遺伝子発現
Outline of Final Research Achievements	In this study, we generated a tetracycline-inducible FoxO1 expressing keratinocyte cell line (Tet-On Ty1-FoxO1 cells), in order to clarify the functional roles of FoxO1 in keratinocytes. Tet-On Ty1-FoxO1 cells were treated with doxycycline to induce the expression of FoxO1, and then subjected to a global gene expression analysis. We identified FoxO1-regulated genes in keratinocytes, and confirmed the FoxO1 binding to genomic DNA of the target genes. Furthermore, we found that 3-deazaneplanocin A reduced the protein levels of FoxO1 in an oral squamous cell carcinoma cell line.

Report

(4 results)