Elucidation of the functional roles of Ca2+-activated potassium channels in osteoclastic bone resorption
| Project/Area Number |
25462907
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
OKAMOTO FUJIO 福岡歯科大学, 歯学部, 講師 (60153938)
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| Co-Investigator(Kenkyū-buntansha) |
KAJIYA Hiroshi 福岡歯科大学, 口腔歯学部, 講師 (80177378)
OKABE Koji 福岡歯科大学, 口腔歯学部, 教授 (80224046)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 骨代謝 / 破骨細胞 / カルシウム活性化カリウムチャネル / ＴＲＰチャネル / 骨吸収 / TRPチャネル / アクチンリング / 非選択的陽イオンチャネル |
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| Outline of Final Research Achievements |
We investigated the functional role of Ca2+-activated K+ channels (Kca) in osteoclastic bone resorption. Mature osteoclasts derived from mouse bone marrow cells abundantly expressed Kca 3.1. Pharmacological inhibition of Kca 3.1 led to rapid disappearance of ring-like actin conformation (actin-ring) and reduced bone resorption activity. Other Kca blockers had no effect on both the actin-ring conformation and the bone resorption activity. Mature osteoclasts also expressed non-selective cation channels including TRPM7. Inhibition of these cation channels also reduced the bone resorption activity. These results suggest that Kca 3.1 expressed in mature osteoclasts serves as a regulator of Ca2+ influx through the non-selective cation channels, which contribute to the formation of actin-ring, an essential process for the bone resorption.
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Report
(4 results)
Research Products
(5 results)
