| Project/Area Number |
25462918
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
YOSHIDA Kaya 徳島大学, 大学院医歯薬学研究部, 講師 (60363157)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMURA Hirohiko 徳島大学, 大学院医歯薬学研究部, 准教授 (20380024)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | PKR / インフラマソーム / 歯周病 / 骨芽細胞 / NLRP3 / NF-kappaB / priming / 破骨細胞活性化 / ＰＫＲリン酸化 / 炎症性サイトカイン / 骨代謝調節因子 |
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| Outline of Final Research Achievements |
To investigate the roles of PKR and NLRP3 inflammasome on the progress of periodontal diseases, we examine whether PKR affect NLRP3 and bone metabolisms in P. gingivalis-infected osteoblasts.
P. gingivalis increased the expression and phosphorylation of PKR in mouse osteoblasts, MC3T3-E1. P. gingivalis also induced and activated NLRP3, resulting in the increase of caspase-1 cleavage and interleukin (IL)-1b release. PKR was necessary to the up-regulation of NLRP3. Moreover, P. gingivalis affects the expression of several bone-related genes through by PKR-dependent pathway. These results showed that PKR mediates bone metabolisms by affecting NLRP3 and bone-related genes in periodontal diseases.
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