| Project/Area Number |
25462925
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Nagasaki University |
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Principal Investigator |
YUKITAKE Hideharu 長崎大学, 医歯薬学総合研究科(歯学系), 技術職員 (30380984)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAYAMA Koji 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (80150473)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 歯周病細菌 / 分泌調節メカニズム / ９型分泌機構 / 二成分制御系 / ECFシグマ因子 / ゲルシフトアッセイ / 9型分泌機構 / 国際情報交換 / アリゾナ州 / SILAC法 / RT-PCR法 / フォスファターゼ活性 / ゲルシフト解析 / プライマーイクステンション / フィンガープリント法 |
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| Outline of Final Research Achievements |
Porphyromonas gingivalis secretes potent pathogenic proteases, gingipains, via the type IX secretion system (T9SS). We found that the PorY-PorX-SigP cascade is involved in the regulation of T9SS. Surface plasmon resonance (SPR) analysis revealed a direct interaction between PorY and PorX. PorY autophosphorylated and transferred a phosphoryl group to PorX in the presence of Mn2+, demonstrating that PorX and PorY act as a response regulator and a histidine kinase, respectively, of a two component system (TCS). T9SS component-encoding genes were down-regulated in a mutant deficient in a putative extracytoplasmic function (ECF) sigma factor, SigP, similar to the porX mutant. SigP could bind to the putative promoter regions of T9SS component-encoding genes. SigP was lacking in the porX mutant. PorX could bind to SigP. Together, these results indicate that the PorXY TCS regulates T9SS-mediated protein secretion via the SigP ECF sigma factor.
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