| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Outline of Final Research Achievements |
We developed detection systems for EGFR-1 and KRAS gene mutation. In addition, we attempted detection of these gene mutations from oral swab specimen.
We designed primer sets for each gene mutations, and investigate several cell lines. The result showed that the system detected gene mutations within 20 minutes. However, oral swab failed detection of these gene mutations. We simulate the number and preparation conditions in cell line, which resulted lower detection limit (10-100 cells). These data indicate that there are very few mutation positive cases or it is difficult to detect from keratinized cells.
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