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Role of cancer stem cells and epithelial-mesenchymal transition in the invasion and metastasis in oral squamous cell carcinoma

Research Project

Project/Area Number 25462940
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionAichi Gakuin University

Principal Investigator

Goto Mitsuo  愛知学院大学, 歯学部, 講師 (60645191)

Co-Investigator(Kenkyū-buntansha) 中西 速夫  愛知県がんセンター(研究所), その他部局等, その他(移行) (20207830)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords口腔扁平上皮がん / 低分化がん / CSC/EMT / podoplanin / TGF-β / PI3K/Akt経路 / 分子標的薬 / EMT/CSC / シグナル伝達 / Wnt/beta-catenin経路
Outline of Final Research Achievements

Cancer stem cells (CSC) and epithelial-mesenchymal transition (EMT) are reportedly involved in tumor invasion and metastasis in oral squamous cell carcinoma (OSCC). However, molecular base of this relationship remains unclear. Here, we focused on podoplanin (PDPN) to investigate its role in cancer stemness and EMT of OSCC. We developed a panel of UMSCC81B cell lines including parent cells, CSC subline, and EMT subline.
PDPN expression of parent and CSC cells was significantly higher than EMT cell. In vitro analysis revealed that PDPN expression of parent and CSC cells was down-regulated by HDAC inhibitors, whereas expressions of differentiation markers were up-regulated by the HDAC treatment. Knockdown of PDPN induced differentiation and suppressed cell growth. TGF-β induced PDPN expression. These results suggest that PDPN plays some important role in maintaining cancer stemness but not maintenance of EMT phenotype. PDPN would be a new therapeutic target for cancer stem cell of OSCC.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (3 results)

All 2017 2015 2014

All Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] 口腔扁平上皮癌におけるPodoplanin発現とがん幹細胞形質との関連に関する実験的検討2017

    • Author(s)
      後藤満雄、宮地 斉、落合栄樹、齋藤輝海、渡邉 哲、宮部 悟、欄 真一郎、中西速夫、下郷和雄
    • Organizer
      第35回日本口腔腫瘍学会総会・学術大会
    • Place of Presentation
      福岡県 福岡市
    • Year and Date
      2017-01-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] Role of podoplanin expression and its potential as a targeting molecule for oral squamous cell carcinomas2015

    • Author(s)
      Mitsuo Goto, Hiroyuki Makihara, Mitsuhiko Ohta, Shinichiro Maseki, Yasuhisa Hasegawa, Kenji Yoshida, Hayao Nakanishi, Kenichi Kurita
    • Organizer
      22nd International Conference on Oral and Maxillofacial Surgery
    • Place of Presentation
      Melbourne, Australia
    • Year and Date
      2015-10-27
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Biological significance and regulation of podoplanin expression in oral squamous cell carcinomas.2014

    • Author(s)
      Mitsuo Goto, Hiroyuki Makihara, Mitsuhiko Ohta, Shinichiro Maseki, Yasuhisa Hasegawa, Hayao Nakanishi, Kenichi Kurita
    • Organizer
      American Association of Oral and Maxillofacial Surgeons, 96th Annual Meeting, Scientific Sessions & Exhibition
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2014-09-08 – 2014-09-13
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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