Influence of non-enzymatic glycation in human dentin on aging
| Project/Area Number |
25462957
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
Miura Jiro 大阪大学, 歯学部附属病院, 助教 (70437383)
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| Co-Investigator(Kenkyū-buntansha) |
Fukushima Shuichiro 大阪大学, 基礎工学研究科, 助教 (40362644)
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| Co-Investigator(Renkei-kenkyūsha) |
Araki Tsutomu 大阪大学, 基礎工学研究科, 教授 (50136214)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 加齢 / 象牙質 / コラーゲン / 糖化 / AGEｓ / 老化 / AGE |
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| Outline of Final Research Achievements |
The main purpose of this study was to determine the Maillard reaction in dentin. We focused on the crosslinking of collagen; namely, advanced glycation end products (AGEs) formed by non-enzymatic glycosylation. Fluorescence signals, electrophoresis, and Western blotting can be used as probes for this research. In this study, we examined the relationship between fluorescence characteristics and immunochemical analysis of type1 collagen from decalcified human dentin with respect to glycation, and considered the possible influence of crosslinking on dentinal physiology.
The accumulation of AGEs with aging may be responsible for the changes in fluorescence lifetime and immunochemical properties. Fluorescence microscopy and electrophoresis can provide useful crosslinking information on the aging phenomenon in human dentin. These results suggest that dentinal aging might be affected by reducing sugars in the blood.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Comparative Analysis of Mouse Induced Pluripotent Stem Cells and Mesenchymal Stem Cells during Osteogenic Differentiation in vitro.2014
Author(s)
Egusa, H., Kayashima, H., Miura, J., Uraguchi, S., Wang, F., Okawa, H., Sasaki, J.I., Saeki, M., Matsumoto, T., and Yatani, H.
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Journal Title
Stem cells and development.
Volume: 23
Issue: 18
Pages: 2156-2169
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Basolateral Mg2+ extrusion via CNNM4 mediates transcellular Mg2+ transport across epithelia: a mouse model2013
Author(s)
Daisuke Yamazaki, Yosuke Funato, Jiro Miura, Sunao Sato, Satoru Toyosawa, Kazuharu Furutani, Yoshihisa Kurachi, Yoshihiro Omori, Takahisa Furukawa, Tetsuya Tsuda, Susumu Kuwabata, Shin Mizukami, Kazuya Kikuchi, Hiroaki Miki
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Journal Title
PLoS Genetics
Volume: 9(12)
Issue: 12
Pages: e1003983-e1003983
DOI
Related Report
Peer Reviewed
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