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Influence of non-enzymatic glycation in human dentin on aging

Research Project

Project/Area Number 25462957
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Conservative dentistry
Research InstitutionOsaka University

Principal Investigator

Miura Jiro  大阪大学, 歯学部附属病院, 助教 (70437383)

Co-Investigator(Kenkyū-buntansha) Fukushima Shuichiro  大阪大学, 基礎工学研究科, 助教 (40362644)
Co-Investigator(Renkei-kenkyūsha) Araki Tsutomu  大阪大学, 基礎工学研究科, 教授 (50136214)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords加齢 / 象牙質 / コラーゲン / 糖化 / AGEs / 老化 / AGE
Outline of Final Research Achievements

The main purpose of this study was to determine the Maillard reaction in dentin. We focused on the crosslinking of collagen; namely, advanced glycation end products (AGEs) formed by non-enzymatic glycosylation. Fluorescence signals, electrophoresis, and Western blotting can be used as probes for this research. In this study, we examined the relationship between fluorescence characteristics and immunochemical analysis of type1 collagen from decalcified human dentin with respect to glycation, and considered the possible influence of crosslinking on dentinal physiology.
The accumulation of AGEs with aging may be responsible for the changes in fluorescence lifetime and immunochemical properties. Fluorescence microscopy and electrophoresis can provide useful crosslinking information on the aging phenomenon in human dentin. These results suggest that dentinal aging might be affected by reducing sugars in the blood.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (20 results)

All 2015 2014 2013

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (12 results) (of which Invited: 2 results)

  • [Journal Article] Simple Observation of Streptococcus mutans Biofilm by Scanning Electron Microscopy using Ionic Liquids2015

    • Author(s)
      Asahi Y, Miura J, Tsuda T,et al
    • Journal Title

      AMB Express

      Volume: 5(1): Pages: 6-6

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Melatonin Inhibits Embryonic Salivary Gland Branching Morphogenesis by Regulating Both Epithelial Cell Adhesion and Morphology.2015

    • Author(s)
      Obana-Koshino A, Ono H, Miura J, Sakai M, Uchida H, Nakamura W, Nohara K, Maruyama Y, Hattori A, Sakai T.
    • Journal Title

      PLoS One

      Volume: 10(4) Issue: 4 Pages: e0119960-e0119960

    • DOI

      10.1371/journal.pone.0119960

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Decrease in fluorescence lifetime by glycation of collagen and its application in determining advanced glycation end-products in human dentin2015

    • Author(s)
      Shuichiro Fukushima, Masato Shimizu, Jiro Miura, Yusuke Matsuda, Mizuho Kubo, Mamoru Hashimoto, Takuya Aoki, Fumio Takeshige, and Tsutomu Araki
    • Journal Title

      Biomedical Optics Express

      Volume: 6 Issue: 5 Pages: 1844-1856

    • DOI

      10.1364/boe.6.001844

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Accumulation of advanced glycation end-products in human dentin by aging2014

    • Author(s)
      Jiro Miura, Kantaro Nishikawa, Mizuho Kubo, Shuichiro Fukushima, Mamoru Hashimoto, Fumio Takeshige, and Tsutomu Araki
    • Journal Title

      Arch Oral Biol

      Volume: Vol. 59, No. 2 Issue: 2 Pages: 119-124

    • DOI

      10.1016/j.archoralbio.2013.10.012

    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of the tea catechin epigallocatechin gallate on Porphyromonas gingivalis biofilms2014

    • Author(s)
      Asahi Y. et al.
    • Journal Title

      Jouranl of Applied Microbiology

      Volume: 116 Issue: 5 Pages: 1164-1171

    • DOI

      10.1111/jam.12458

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparative Analysis of Mouse Induced Pluripotent Stem Cells and Mesenchymal Stem Cells during Osteogenic Differentiation in vitro.2014

    • Author(s)
      Egusa, H., Kayashima, H., Miura, J., Uraguchi, S., Wang, F., Okawa, H., Sasaki, J.I., Saeki, M., Matsumoto, T., and Yatani, H.
    • Journal Title

      Stem cells and development.

      Volume: 23 Issue: 18 Pages: 2156-2169

    • DOI

      10.1089/scd.2013.0344

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Basolateral Mg2+ extrusion via CNNM4 mediates transcellular Mg2+ transport across epithelia: a mouse model2013

    • Author(s)
      Daisuke Yamazaki, Yosuke Funato, Jiro Miura, Sunao Sato, Satoru Toyosawa, Kazuharu Furutani, Yoshihisa Kurachi, Yoshihiro Omori, Takahisa Furukawa, Tetsuya Tsuda, Susumu Kuwabata, Shin Mizukami, Kazuya Kikuchi, Hiroaki Miki
    • Journal Title

      PLoS Genetics

      Volume: 9(12) Issue: 12 Pages: e1003983-e1003983

    • DOI

      10.1371/journal.pgen.1003983

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structural modifications of dentinal microcracks with human aging2013

    • Author(s)
      Mizuho Kubo, Jiro Miura, Takao Sakata, Ryuji Nishi and Fumio Takeshige
    • Journal Title

      Microscopy

      Volume: 62 Issue: 6 Pages: 555-561

    • DOI

      10.1093/jmicro/dft028

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ナノインデンテーションにおける象牙質コラーゲン線維の物性評価2015

    • Author(s)
      久保 美寿穂、三浦 治郎、松中 大介、田中 展、竹重 文雄、渋谷 陽二
    • Organizer
      日本真空学会 機能薄膜部会ナノ・キャラクタリゼイション専門部会 第5回研究会
    • Place of Presentation
      東陽テクニカ会議室
    • Year and Date
      2015-10-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 直交配置型FIB-SEMによる象牙質疾患の未脱灰3次元超微形態観察2015

    • Author(s)
      三浦 治郎、清水 真人、榎本 早希子、荒井 重勇
    • Organizer
      歯科基礎医学会
    • Place of Presentation
      朱鷺メッセ 新潟市
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 象牙質コラーゲンの糖化と加齢変化2015

    • Author(s)
      清水 真人,三浦 治郎
    • Organizer
      歯科基礎医学会
    • Place of Presentation
      朱鷺メッセ 新潟市
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 未脱灰象牙質における3次元構築法2014

    • Author(s)
      三浦治郎
    • Organizer
      歯科基礎医学会
    • Place of Presentation
      福岡市
    • Year and Date
      2014-09-24 – 2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 象牙質基質タンパク質の糖化による加齢変化2014

    • Author(s)
      清水真人 三浦治郎
    • Organizer
      歯科基礎医学会
    • Place of Presentation
      福岡市
    • Year and Date
      2014-09-24 – 2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] Determination of Advanced Glycation End-products in human dentin.2014

    • Author(s)
      M. Shimizu, J. Miura, M. Kubo, Y. Matsuda, S. Fukushima, F. Takeshige, and T. Araki,
    • Organizer
      IADR/PER
    • Place of Presentation
      Dubrobnik, Croatia
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] 齲蝕罹患象牙質における基質蛋白の多面的評価2014

    • Author(s)
      松田祐輔、三浦治郎、清水真人、久保美寿穂、竹重文雄、坂田孝夫 西竜治
    • Organizer
      第30回 医学生物学電子顕微鏡技術学会
    • Place of Presentation
      吹田市
    • Year and Date
      2014-05-24 – 2014-05-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Distribution of Advanced glycation end-products in human dentin measured by fluorescence lifetime imaging2013

    • Author(s)
      Nishikawa Kantaro, Fukushima Shuichiro, Araki Tsutomu, Kubo Mizuho, Miura Jiro, Takeshige Fumio
    • Organizer
      The 15th International Conference on Biomedical Engineering
    • Place of Presentation
      National University of Singapore
    • Related Report
      2013 Research-status Report
  • [Presentation] 硬いサンプルを切ってみる -未脱灰硬組織への挑戦2013

    • Author(s)
      三浦治郎
    • Organizer
      第35回医学生物学電子顕微鏡学会近畿支部会
    • Place of Presentation
      奈良先端科学技術大学院大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 類骨における石灰化部位と骨細胞の超微構造学的解析2013

    • Author(s)
      三浦 治郎 大家香織 佐藤 淳 豊澤 悟
    • Organizer
      日本歯科基礎医学会
    • Place of Presentation
      岡山コンベンションセンター
    • Related Report
      2013 Research-status Report
  • [Presentation] 加齢に伴う象牙細管近傍におけるAGEs蓄積の観測2013

    • Author(s)
      西川貫太郎、福島修一郎、荒木勉 三浦治郎
    • Organizer
      応用物理学会関西支部
    • Place of Presentation
      奈良市
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒト象牙質における微小亀裂進展とコラーゲン線維の加齢変化2013

    • Author(s)
      久保美寿穂、三浦治郎、清水真人、松田祐輔、長島正、竹重文雄
    • Organizer
      第139回日本歯科保存学会
    • Place of Presentation
      秋田市
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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