Preparation of osteo-conductive device using nano-size apatite particles

• Project/Area Number: 25463013
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Iwate Medical University
• Principal Investigator: Taira Masayuki 岩手医科大学, 歯学部, 准教授 (60179398)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 生体材料 / ナノアパタイト / コラーゲン / 複合体 / 硬組織再生 / 医療デバイス / 架橋型ヒアルロン酸 / BMP / 注入型 / 硬組織形成 / 合成コラーゲン / ポリ乳酸共重合体 / コラーゲン複合体

Outline of Final Research Achievements

We prepared collagen/nano-apatite composites by blending, and implanted them in rat cranial defects. It became evident that sponge composites were absorbed, but pressed composites had good osteo-conductive properties, leading to much new bone formation. It was suggested that pressed collagen/nano-apatite composites are new bone substitute materials.
We also prepared crosslink-type hyaluronic acid/nano-apatite/BMP sols, and implanted them in mouse head, back and muscle. The materials (gel) induced bone formation at high rates. It was speculated that crosslink-type hyaluronic acid/nano-apatite/BMP composite might be used as safe injectable (sol-gel reaction type) osteo-cunductive medical device.

Research Products

• [Journal Article] In vivo evaluation of noble porous apatite disks implanted in rat critical-size calvarial defects by Micro-CT and histological observations. (2014)
  • Author(s): Hatakeyama W, Taira M, Ikeda K, Takafuji K, Kihara H, Kondo H, Hattori M.
  • Journal Title: J Oral Tissue Eng. Volume: 12
  • NAID: 130005130405
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Tracking GFP-labeled transplanted mouse MSC in nude mice using in vivo fluorescence imaging (2014)
  • Author(s): Taira M, Hatekeyama W, Yokota J, Chosa N, Ishisaki A, Takafuji K, Kihara H, Kondo H, Hattori M.
  • Journal Title: Nano Biomed. Volume: 6
  • NAID: 130005130560
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Novel SCRG1/BST1 axis regulates self-renewal, migration, and osteogenic differentiation potential in mesenchymal stem cells. (2014)
  • Author(s): Aomatsu E., Takahashi N., Sawada S, Okubo N, Hasegawa T, Taira M, Miura H, Ishisaki A, Chosa N.
  • Journal Title: Scientific Reports Volume: 4 Pages: 3652-3652
  • Peer Reviewed
• [Journal Article] Bone-regeneration trial of rat critical-size calvarial defects using nano-apatite/collagen composites. (2013)
  • Author(s): Hatakeyama W, Taira M, Takafuji K, Kihara H, Kondo H.
  • Journal Title: Nano Biomed. Volume: 5 Pages: 95-103
  • NAID: 130004679861
  • Peer Reviewed
• [Journal Article] Effects of apatite particle size contained in two apatite/collagen composites on osteogenic differentiation profile in osteoblastic cells. (2013)
  • Author(s): Hatakeyama W., Taira M., Chosa N., Kihara H., Ishisaki A., Kondo H.
  • Journal Title: International Journal of Molecular Medicine Volume: 32 Issue: 6 Pages: 1255-1261
  • DOI: 10.3892/ijmm.2013.1516
  • Peer Reviewed
• [Presentation] ラット頭蓋骨欠損部におけるプレス加工ナノ・アパタイト/コラーゲン複合体を用いた骨再生 (2015)
  • Author(s): 池田功司，平 雅之，高藤恭子，近藤尚知．
  • Organizer: 第45回口腔インプラント学会学術大会
  • Place of Presentation: 岡山市
  • Year and Date: 2015-09-22
• [Presentation] ラット頭蓋骨骨欠損部におけるハイドロキシアパタイト多孔体ディスクを用いた骨再生の試み (2014)
  • Author(s): 畠山 航, 平 雅之, 池田功司, 鬼原英道, 近藤 尚知
  • Organizer: 第44回日本口腔インプラント学会
  • Place of Presentation: 東京都
  • Year and Date: 2014-09-13
• [Presentation] ナノアパタイト／コラーゲン複合体に対する生体反応の評価：骨芽細胞応答、軟組織反応および硬組織反応．
  • Author(s): 畠山 航，平 雅之，鬼原英道，近藤尚知．
  • Organizer: 日本歯科理工学会 北海道東北地方会夏期セミナー
  • Place of Presentation: 岩手県盛岡市
• [Presentation] アパタイト/コラーゲン複合体中のアパタイト粒子の違いが骨芽細胞様細胞の骨系分化挙動に及ぼす影響評価．
  • Author(s): 畠山航，平 雅之，鬼原英道，髙藤恭子，近藤尚知．
  • Organizer: 第43回日本口腔インプラント学会学術大会．
  • Place of Presentation: 福岡県福岡市
• [Presentation] 多孔質アパタイト粒子コラーゲン複合体によるラット頭蓋骨クリティカルサイズ骨欠損部における骨再生の試み．
  • Author(s): 畠山 航，平 雅之，鬼原 英道，近藤 尚知．
  • Organizer: 第62回日本歯科理工学会学術講演会．
  • Place of Presentation: 新潟県新潟市
• [Remarks] 岩手医科大学 医療工学講座のホームページにようこそ
  • URL: http://hitech-d.iwate-med.ac.jp/dmst/index-j.htm
• [Remarks] http://hitech-d.iwate-med.ac.jp/dmst/lab/lab.htm
• [Remarks] 岩手医科大学 医療工学講座のホームページにようこそ
  • URL: http://hitech-d.iwate-med.ac.jp/dmst/index-j.htm