| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The purpose of this study is to investigate the surface characteristics and biocompatibility of titanium (Ti) surfaces modified by wire electrical discharge machining (EDM). EDM surface characteristics were evaluated by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), thin-film X-ray diffractometry (XRD) and contact angle measurements. The bone substance were evaluated by nano-indentation test. EDM surfaces exhibited high super hydrophilicity, due to high surface energy. XPS and XRD revealed that a passive oxide layer with certain developing thickness onto. EDM surfaces promoted cell attachment, but restrained proliferation. Real-time PCR analyses showed significantly higher relative mRNA expression levels of osteoblastic genes in cells cultured on the EDM surfaces as compared to cells cultured on the machined surfaces and the bone substance on the EDM surfaces was equivalent to cortical bone.
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