Regulation of AChR cluster formation by miR-206 in masticatory muscles of mi/mi mice.

• Project/Area Number: 25463037
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Tsurumi University
• Principal Investigator: SUGA Takeo 鶴見大学, 歯学部, 講師 (40247333)
• Co-Investigator(Kenkyū-buntansha): OGAWA Takumi 鶴見大学, 歯学部, 教授 (20267537) ; ANDO Hitoshi 鶴見大学, 歯学部, 講師 (00282765) ; 山根 明 鶴見大学, 歯学部, 教授 (20166763)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: miR-206 / miR-29 / mi/mi mouse / 咬筋 / アセチルコリン受容体 / miR-29a / 非咬合マウス / mi/miマウス / ACh受容体 / Ach受容体

Outline of Final Research Achievements

Expression of endogenous miR-206 markedly increased with a peak at 4 weeks in the masseter of wild mice, but it kept low level until 12 weeks in those of the mi/mi mice. It suggested that miR-206 has a role in the formation of ACh receptor clusters. However, The average number of ACh receptor clusters was not changed when expression of miR-206 was inhibited or enhanced. More successful method of loss and gain of function experiments for microRNAs. We also investigated expression change of miR-29a which is another important microRNA in mygenesis. Expression pattern of miR-29a was similar to miR-206, but it could not change expressions of Agrin, MuSK, Lrp4 and Rapsin, which are regulation genes of ACh reseptor cluster formation.

Research Products

• [Journal Article] MyoD is Regulated by the miR-29a-Tet1 Pathway in C2C12 Myoblast Cels (2016)
  • Author(s): Chikenji A, Ando H., Nariyama M, Suga T, Iida R, Gomi K
  • Journal Title: Journal of Oral Science Volume: 58
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Functions of miR-1 and miR-133a during the postnatal development of masseter and gastrocnemius muscles. (2015)
  • Author(s): Nariyama M, Mori M, Shimazaki E, Ando H, Ohnuki Y, Abo T, Yamane A, Asada Y
  • Journal Title: Mol Cell Biochem Volume: 404 Issue: 1-2 Pages: 17-27
  • DOI: 10.1007/s11010-015-2450-y
  • Peer Reviewed
• [Journal Article] Function of miR-1 in the differentiation of mouse tongue striated muscle cell (2014)
  • Author(s): MAKUUCHI, S., FUKUI, T., YAMANE, A. OHKUBO, C.
  • Journal Title: Tsurumi Dent. J. In press Volume: In press
  • Peer Reviewed
• [Journal Article] Role of occlusion in mouse masseter muscle acetylcholine receptor clustering (2013)
  • Author(s): MORI, M., NARIYAMA, M., ABO, T., HIRAI, S., OGAWA, T., HAMADA, Y., YAMANE, A. and ASADA, Y.
  • Journal Title: J. Dent. Res. Volume: 92 Issue: 4 Pages: 352-357
  • DOI: 10.1177/0022034513476038
  • Peer Reviewed
• [Journal Article] Age-related changes in rat genioglossus, geniohyoid, and masseter muscles (2013)
  • Author(s): KANEKO, S., IIDA, R., SUGA, T., MORITO, M. and YAMANE, A.
  • Journal Title: Gerodontology Volume: 未定 Issue: 1 Pages: 56-62
  • DOI: 10.1111/ger.12004
  • Peer Reviewed
• [Presentation] 咬合様式の違いが咬筋の神経筋接合部におけるアセチルコリン受容体クラスター形成および運動神経分布に及ぼす影響 (2013)
  • Author(s): 森 愛美
  • Organizer: 小児歯科学会
  • Place of Presentation: 長良川国際会議場